02717naa a2200457 a 450000100080000000500110000800800410001902400590006010000220011924501800014126000090032152013430033065000130167365000280168665000270171465000090174165000120175065000100176265000170177265000190178965000170180865000170182565000280184265000120187065000100188265300280189265300340192065300080195465300310196265300170199365300230201070000190203370000240205270000160207670000250209270000180211770000190213570000220215470000230217677300600219921608922024-03-06 2024 bl uuuu u00u1 u #d7 ahttps://doi.org/10.1016/j.anireprosci.2023.1073962DOI1 aGONÇALVES, J. D. aLuteal tissue characteristics of Morada Nova ewes with hCG application 7.5 days after the end of estrus synchronization protocol in the breeding season.h[electronic resource] c2024 aAbstract: Results with the use of hCG after synchronization protocol are still inconsistent, which may vary according to breed, season, day of application and dose of the drug used. The aim of the present study was to evaluate the functionality of luteal tissue and ovarian perfusion after hCG treatment during early luteal phase. Estrus-synchronized ewes were randomly assigned to receive i.m. injection of 300 IU of hCG (G-hCG; n = 40) or 1 mL of saline (G-Control; n = 32) on Day 7.5 after progesterone withdrawal. Ultrasonographic evaluations of the ovaries and ovarian and iliac arteries were performed on Days 7.5, 10.5, 13.5, and 21.5. The accessory corpus luteum (aCL) formation rate was 52.5% for G-hCG. There was interaction (p > 0.05) for treatment (G-hCG and G-Control), days (7.5, 10.5, 13.5 and 21.5) and PD (Pregnant and Non-pregnant) for the variables of biometric characteristics of the corpus luteum B-Mode and Color Doppler on days 7.5, 10.5, 13.5 and 21.5. There was no difference (p > 0.05) for pregnancy rates and mean fetuses per ewe between the treatment groups. It is concluded that the application of hCG 7.5 days after the hormonal protocol in Morada Nova ewes in a breeding season is efficient in inducing aCL formation and increasing luteal tissue biometry. However, there was no effect on pregnancy rate. aBreeding aChorionic gonadotropins aEstrus synchronization aEwes aFemales aMales aPharmacology aPregnancy rate aProgesterone aReproduction aReproductive efficiency aSeasons aSheep aAccessory corpora lutea aArterial velocimetric indices aHCG aLuteal color Doppler signs aNon-breeding aOvarian ultrasound1 aVERGANI, G. B.1 aRODRIGUES, J. N. D.1 aDIAS, J. H.1 aPEREIRA, V. S. do A.1 aGARCIA, A. R.1 aESTEVES, S. N.1 aFONSECA, J. F. da1 aOLIVEIRA, M. E. F. tAnimal Reproduction Sciencegv. 261, 107396, Feb. 2024.