01508naa a2200217 a 450000100080000000500110000800800410001902400520006010000170011224501640012926000090029352007560030265000170105865000270107565000430110265000170114570000240116270000220118670000210120877300610122921605622024-01-08       2010    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1016/j.ab.2010.03.0382DOI1 aCARVALHO, K.  aReference gene selection for real-time quantitative polymerase chain reaction normalization in "Swingle" citrumelo under drought stress.h[electronic resource]  c2010  aWe describe the first systematic evaluation of reference genes for use in real-time quantitative polymerase chain reaction (qPCR) for water deficit stress studies in the citrus rootstock "Swingle" citrumelo. The expression levels of seven reference genes cyclophilin (CYP), cathepsin (CtP), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1 alpha), beta-tubulin (TUB), and ADP ribosylation factor (ADP) during drought stress were tested using geNorm and NormFinder programs. Results from four experimental conditions indicated that EF1 alpha and ADP were the most stable reference genes. Relative expression levels of Delta 1-pyrroline-5-carboxylate synthetase (P5CS) was used for reference gene validation.  aCyclophilins  aPlant selection guides  aQuantitative polymerase chain reaction  aWater stress1 aCAMPOS, M. K. F. de1 aPEREIRA, L. F. P.1 aVIEIRA, L. G. E.  tAnalytical Biochemistrygv. 402, n. 2, p. 197-199, 2010.