02234naa a2200289 a 450000100080000000500110000800800410001902400520006010000200011224501800013226000090031250000330032152013100035465000190166465000180168365000200170165300090172165300260173065300090175665300170176565300370178270000230181970000190184270000170186170000240187877300420190221603662024-01-17       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s11947-023-03297-62DOI1 aTORRES NETO, L.  aNanoemulsification of Essential Oil Blend by UltrasoundbOptimization of Physicochemical, Antioxidant Properties, and Activity Against Escherichia coli.h[electronic resource]  c2023  aPublished: 16 December 2023.  aThis study aimed to select the best conditions for producing a nanoemulsion loaded with an essential oil blend (NEOB) to achieve maximum antimicrobial and antioxidant activities. The NEOB consisted of lemongrass (Cymbopogon citratus; LEO; 60%) and thyme (Thymus vulgaris; TEO; 40%). The effect of ultrasound time exposition at 300 W (5, 18, and 30 min) and essential oil concentration (5, 11, and 20 mg/mL) on these responses was evaluated after obtaining NEOB and after an oxidation assay with UVC-LED exposure. Nanoemulsions were analyzed for their physicochemical and biological properties. Overall, droplet size, zeta potential, minimum inhibitory concentrations (MIC) against Escherichia coli, DPPH free radical scavenging activity, and ferric-reducing antioxidant power were affected by essential oil concentration and/or ultrasound time exposition in a non-linear fashion. The NEOB (average size, 68.88 ± 2.84 nm) with 20.5 mg/mL of essential oil and sonicated for 35.7 min achieved the highest anti-E. coli (MIC, 0.44 mg/mL) and antioxidant activity (FRAP, 1.17 μmol TROLOX equivalent/g), remaining stable even after high UVC-LED doses (6.41 J/cm2 exposure, p &lt 0.05). This study reveals the promising potential of NEOB obtained from ultrasound for sustainable industrial applications.  aEssential oils  aNanoemulsions  aÓleo Essencial  aCCRD  aDesirability function  aFRAP  aNanoemulsão  aUltraviolet light-emitting diode1 aMONTERIO, M. L. G.1 aMUTZ, U. DA S.1 aTONON, R. V.1 aCONTE-JUNIOR, C. A.  tFood and Bioprocess Technology, 2023.