01990nam a2200265 a 450000100080000000500110000800800410001910000230006024501890008326002180027250000120049052009920050265000120149465000220150665000290152865000220155765000250157965000180160465000120162265000110163470000170164570000180166270000200168070000240170021603012023-12-28       2023    bl uuuu     u00u1 u    #d1 aCARVALHO, B. A. R.  aProduction of cellulolytic and hemicellulolytic enzymes by the pectinolytic Aspergillus niger 3T5B8-C88-P83 mutant strain under different cultivation conditions.h[electronic resource]  aIn: INTERNATIONAL SYMPOSIUM OF MICROBIOLOGY AND BIOTECHNOLOGY, 6., 2023, Viçosa, MG. Biotecnologia Microbiana Soluções para os grandes desafios globais: anais. Viçosa, MG: Universidade Federal de Viçosac2023  aResumo.  aEmbrapa Agroenergia has been conducting genetic improvement of filamentous fungi to increase the production of cellulases and hemicellulases to deconstruct lignocellulosic biomass. In a conventional breeding program for Aspergillus niger 3T5B8 performed in 2015, after two rounds of mutagenesis (ultraviolet followed by ethyl methanesulfonate), the pectinolytic mutant strain 3T5B8-C88-P83 was selected, which presents genetic changes in the catabolite repression by glucose pathway and a significant increase in polygalacturonase production compared to the original 3T5B8 strain. This work aimed to evaluate the production of cellulolytic and hemicellulolytic enzymes of the 3T5B8-C88-83 mutant under different cultivation conditions, seeking its future application in the enzymatic processing of cotton fabrics. Endoglucanase production by the 3T5B8-C88-P83 mutant strain was evaluated in 250 mL Erlenmeyer flasks containing different liquid and solid culture media (four repetitions).  aMutants  aPolygalacturonase  aSolid state fermentation  aAspergillus Niger  aEnzima Celulolítica  aFermentação  aMutante  aSolido1 aALVES, L. M.1 aMENDES, T. D.1 aSALUM, T. F. C.1 aFAVARO, L. C. de L.