02273naa a2200277 a 450000100080000000500110000800800410001902400530006010000190011324500740013226000090020630000240021552013380023965000140157765000180159165300280160965300200163765300360165765300280169365300370172170000200175870000170177870000200179570000200181577301600183521594722023-12-13       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/978-981-99-4859-8_62DOI1 aLACORTE, C. C.  aSeed-based production of recombinant proteins.h[electronic resource]  c2023  aCap. 6, p. 185-208.  aThe use of plants to produce recombinant proteins has become a promising alternative to current expression systems based on microorganisms and cell cultures. Producing recombinant proteins in plants presents advantages such as reduced upstream costs, lower probability of infection by mammals’ pathogens, and easy scale-up production. Furthermore, plants can make posttranslational modifications and express large and complex proteins. Seeds stand out for their intrinsic characteristics among the plant tissues that can be used for recombinant protein production. In seeds, recombinant proteins are stored in protein storage vacuoles in the endosperm cells. They can be stably maintained for 4–6 years at room temperature without significant loss of protein and biological activity. Progress in methods for genome editing, alteration of the glycosylation pattern of the recombinant proteins produced, and use of protein fusions to aid downstream processes are some trends that shall contribute further to make molecular farming an attractive option for recombinant protein production. This chapter discusses the potential of seeds as a platform for producing recombinant proteins, across the scale-up of the production systems, basic approaches for the purification of recombinant proteins from plant cells, and biosafety issues  aBiosafety  aGlycosylation  aForeign gene expression  aFusion proteins  aHeterologous protein production  aPlant molecular farming  aRecombinant protein purification1 aFERREIRA, A. L.1 aMURAD, A. M.1 aCUNHA, N. B. da1 aPINHEIRO, P. V.  tIn: KOLE, C.; CHAURASIA, A.; HEFFERON, K. L.; PANIGRAHI, J. (ed.). Tools & techniques of plant molecular farming. [S.l.]: Springer Singapore, 2023. Cap. 6.