01600nam a2200325 a 450000100080000000500110000800800410001910000230006024502010008326001280028430000120041252005070042465000240093165000160095565000260097165000090099765300230100665300260102965300090105570000230106470000200108770000220110770000190112970000220114870000210117070000210119170000220121270000200123470000200125421584932024-04-25       2023    bl uuuu     u01u1 u    #d1 aCOSTA, L. S. de L.  aDepleção do quadro de leitura upstream como nova estratégia para manipular a tradução de gmpr10 usando crispr/cas9 para aumentar a tolerância da soja a fitonematoides.h[electronic resource]  aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais... Florianópolis: SBGc2023  ap. 131.  aIn this study, we identified two uORFs in the 5'-UTR sequence of this gene. The entire 5' leader sequence was amplified, cloned, and site-directed mutated to delete its uORFs start codons (-ATG). The validation of these predicted uORFs was carried out in transformed Nicotiana benthamiana protoplasts using a dual-luciferase reporter vector, in which it is possible to analyze the effect of mutated and non-mutated uORFs by dividing the expression of luciferase by that of Renilla-luciferase (LUC/REN).  aRoot-knot nematodes  aGlycine Max  aMeloidogyne Incognita  aSoja  aEdição do genoma  aNematóide das galhas  aUORF1 aALVES, N. S. de F.1 aPINTO, C. E. M.1 aFLORENTINO, L. H.1 aMELO, B. P. de1 aMOREIRA, V. J. V.1 aSÁ, M. E. L. de1 aARRAES, F. B. M.1 aRECH FILHO, E. L.1 aMORGANTE, C. V.1 aSA, M. F. G. de