02290naa a2200337 a 450000100080000000500110000800800410001902400520006010000210011224501070013326000090024052012820024965000220153165000140155365000200156765000310158765000160161865000170163465000200165165300310167170000200170270000230172270000130174570000210175870000200177970000220179970000200182170000250184170000190186677300670188521575532023-10-26       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s10658-023-02717-92DOI1 aMORAES, G. S. de  aPhylogeny of Pseudoperonospora causing downy mildew of Cucurbitaceae in Brazil.h[electronic resource]  c2023  aDowny mildew of cucurbits is a disease caused by the Oomycete Pseudoperonospora cubensis. It can cause considerable losses in cucurbit crops. Phylogenetic studies point to the existence of cryptic species of Pseudoperonospora. The objective of this study was to determine, the phylogeny of Pseudoperonospora species afecting cucurbits in diferent regions of Brazil. For this, leaves with symptoms of downy mildew were collected in areas of cucurbits production in the states of Pernambuco, Minas Gerais, Ceará, and the Distrito Federal. For phylogenetic analyses, DNA extraction was performed, and PCR reactions were carried out using primers DC-6 and LR-0, Cox2-F and Cox2-RC4, OomCox1-levup and OomCox1-levlo, FM79-C1 and FMPHY-10b, for amplifcation of the internal transcribed spacer (ITS) region, the mitochondrial cytochrome c oxidase subunit II (cox2), the mitochondrial cytochrome c oxidase subunit I (cox1) and cox2-cox1 intergenic spacer respectively. The nucleotide sequences were analyzed, edited, and aligned to perform Bayesian Inference analysis. The results of the phylogenetic analyses presented in the cox2-cox1-cox2_1-ITS concatenated tree indicate that in Brazil, the downy mildew of cucurbits is caused by phylogenetically distinct lineages of P. cubensis.  aGenetic variation  aOomycetes  aPeronosporaceae  aPseudoperonospora cubensis  aWatermelons  aCucumis Melo  aCucumis Sativus  aOomiceto Pseudoperonospora1 aMELLO, J. F. de1 aBRITO, A. C. de Q.1 aREIS, A.1 aMICHEREFF, S. J.1 aGOMES, A. A. M.1 aCÂMARA, M. P. S.1 aVIEIRA, J. C. B1 aSOUZA-MOTA, C. M. de1 aMACHADO, A. R.  tEuropean Journal of Plant Pathologygv. 167, p. 361-370, 2023.