02235naa a2200349 a 450000100080000000500110000800800410001902400490006010000250010924501520013426000090028652011920029565000250148765000180151265000150153065000130154565000120155865000130157065300290158365300220161265300140163465300180164870000140166670000210168070000260170170000270172770000300175470000190178470000220180370000130182577300470183821565072023-09-13       2023    bl uuuu     u00u1 u    #d7 ahttp://doi.org/10.1088/1748-605X/aceac82DOI1 aZANETTE, R. de S. S.  aCytocompatible and osteoinductive cotton cellulose nanofiber/chitosan nanobiocomposite scaffold for bone tissue engineering.h[electronic resource]  c2023  aNatural polymeric nanobiocomposites hold promise in repairing damaged bone tissue in tissue engineering. These materials create an extracellular matrix (ECM)-like microenvironment that induces stem cell differentiation. In this study, we investigated a new cytocompatible nanobiocomposite made from cotton cellulose nanofibers (CNFs) combined with chitosan polymer to induce osteogenic stem cell differentiation. First, we characterized the chemical composition, nanotopography, swelling properties, and mechanical properties of the cotton CNF/chitosan nanobiocomposite scaffold. Then, we examined the biological characteristics of the nanocomposites to evaluate their cytocompatibility and osteogenic differentiation potential using human mesenchymal stem cells derived from exfoliated deciduous teeth. The results showed that the nanobiocomposite exhibited favorable cytocompatibility and promoted osteogenic differentiation of cells without the need for chemical inducers, as demonstrated by the increase in alkaline phosphatase activity and ECM mineralization. Therefore, the cotton CNF/chitosan nanobiocomposite scaffold holds great promise for bone tissue engineering applications.  aCell differentiation  aNanomaterials  aStem cells  aAlgodão  aCélula  aCelulose  aBiomedical nanomaterials  aHuman mesenchymal  aNanofibra  aTecido ósseo1 aFAYER, L.1 aVASCONCELLOS, R.1 aOLIVEIRA, L. F. C. de1 aMARANDUBA, C. M. da C.1 aALVARENGA, E. L. F. C. de1 aMARTINS, M. A.1 aBRANDAO, H. de M.1 aMUNK, M.  tBiomedical Materialsgv. 18, 055016, 2023.