01999naa a2200253 a 450000100080000000500110000800800410001902400370006010000200009724501630011726000090028052012380028965000160152765000090154365300080155265300120156065300140157270000200158670000230160670000250162970000220165470000260167677300430170221561652023-08-28       2023    bl uuuu     u00u1 u    #d7 a10.21577/0100-4042.202300262DOI1 aPORTO, B. L. S.  aRapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID.h[electronic resource]  c2023  aRuminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a flame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency.  aFatty acids  aMilk  aCLA  aFast GC  aSLB IL1111 aFARIA, I. D. L.1 aSOUZA, J. C. Q. DE1 aMOREIRA, O. B. DE O.1 aGAMA, M. A. S. da1 aOLIVEIRA, M. A. L. DE  tQuímica Novagv. 7, p. 743-746, 2023.