02351naa a2200361 a 450000100080000000500110000800800410001902200140006002400480007410000170012224501010013926000090024052013830024965000200163265000190165265000150167165000160168665000100170265000200171265000260173265000130175865000120177165000100178365300140179370000160180770000230182370000240184670000190187070000190188970000170190870000200192577300440194521549582023-07-27       2023    bl uuuu     u00u1 u    #d  a2072-66517 ahttps://doi.org/10.3390/toxins150503442DOI1 aGOMES, T. C.  aEffect of Monocerin, a fungal secondary metabolite, on endothelial cells.h[electronic resource]  c2023  aAbstract: This study reports the isolation and identification of the endophytic fungus Exserohilum rostratum through molecular and morphological analysis using optical and transmission electron microscopy (TEM), as well as the procurement of its secondary metabolite monocerin, an isocoumarin derivative. Considering the previously observed biological activities of monocerin, this study was performed on human umbilical vein endothelial cells (HUVECs) that are widely used as an in vitro model for several different purposes. Important parameters, such as cell viability, senescence-associated ?-galactosidase, cellular proliferation by using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE), apoptosis analysis with annexin, cellular morphology through scanning electron microscopy (SEM), and laser confocal analysis were evaluated after exposing the cells to monocerin. After 24 h of exposure to monocerin at 1.25 mM, there was more than 80% of cell viability and a low percentage of cells in the early and late apoptosis and necrosis. Monocerin increased cell proliferation and did not induce cell senescence. Morphological analysis showed cellular integrity. The study demonstrates aspects of the mechanism of action of monocerin on endothelial cell proliferation, suggesting the possibility of its pharmaceutical application, such as in regenerative medicine.  aCell senescence  aCell viability  aEndophytes  aExserohilum  aFungi  aMedicinal fungi  aSecondary metabolites  aCaatinga  aCróton  aFungo  aMonocerin1 aCONRADO, R.1 aOLIVEIRA, R. C. de1 aSELARI, P. J. R. G.1 aMELO, I. S. de1 aARAÚJO, W. L.1 aMARIA, D. A.1 aSOUZA, A. O. de  tToxinsgv. 15, n. 5, article 344, 2023.