02032naa a2200289 a 450000100080000000500110000800800410001902400520006010000260011224501200013826000090025852011700026765000110143765000190144865000160146765000140148365000190149765000140151670000240153070000200155470000150157470000250158970000280161470000230164270000200166577300570168521549052023-07-13       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s11240-023-02449-y2DOI1 aVILA VERDE, D. DOS S.  aObtaining spontaneous haploids and polyploids from seeds derived from tangerine-type fruits.h[electronic resource]  c2023  aObtaining citrus polyploids, especially from triploid individuals, is of great importance for genetic improvement programs, as they produce seedless fruits. Therefore, the aim of this study was to use flow cytometry analyses to identify polyploid individuals among tangerine-type seedlings originating from seeds germinated in vitro. Fruits were collected from 13 different genotypes in the municipality of Mucugê (state of Bahia, Brazil). The seeds were then removed and classified as developed (seeds with well-formed integument), underdeveloped (seeds with wrinkled integument) and small (seeds of 1/3 to 1/6 of normal size). After the integument was removed, the seeds were disinfected and introduced into a WPM culture medium, in which they remained for 120 days. After this period, the percentage of germinated seeds and growth variables were evaluated, and ploidy analysis was conducted using flow cytometry. The developed seeds displayed a higher germination rate (91.5%) than the others. The small and underdeveloped seeds resulted in a considerable frequency of spontaneous polyploids (11%), and one haploid plant was also obtained from the latter class.  aCitrus  aFlow cytometry  aTetraploidy  aTriploidy  aFruta Cítrica  aTangerina1 aMENDES, M. I. DE S.1 aSOUZA, A. da S.1 aAUD, F. F.1 aSANTOS, K. C. F. dos1 aSOARES FILHO, W. dos S.1 aGESTEIRA, A. da S.1 aCOSTA, M. G. C.  tPlant Cell, Tissue and Organ Culture, january, 2023.