01409naa a2200217 a 450000100080000000500110000800800410001910000170006024500790007726000090015630000150016549000520018052007430023265000100097565000180098565000140100365300200101765300330103770000190107077301020108921542972023-06-06       2023    bl uuuu     u00u1 u    #d1 aSILVA, C. G.  aStem cells as nuclear donors for mammalian cloning.h[electronic resource]  c2023  ap. 115-129  a(Springer. Methods in Molecular Biology, 2647).  aMammals are routinely cloned by introducing somatic nuclei into enucleated oocytes. Cloning contributes to propagating desired animals, to germplasm conservation efforts, among other applications. A challenge to more broader use of this technology is the relatively low cloning efficiency, which inversely correlates with donor cell differentiation status. Emerging evidence suggests that adult multipotent stem cells improve cloning efficiency, while the greater potential of embryonic stem cells for cloning remains restricted to the mouse. The derivation of pluripotent or totipotent stem cells from livestock and wild species and their association with modulators of epigenetic marks in donor cells should increase cloning efficiency.  aClone  aClone Nucelar  aMamífero  aCélulas tronco  aConservação de germoplasma1 aMARTINS, C. F.  tIn: MOURA, M. T. (ed.). Somatic cell nuclear transfer technology. São Paulo: Humana Press, 2023.