02915naa a2200301 a 450000100080000000500110000800800410001902400520006010000200011224501960013226000090032852019830033765000170232065000110233765000190234865000150236765300230238265300200240565300190242565300120244465300170245670000180247370000180249170000130250970000220252270000180254477300510256221537192023-08-22       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s10965-023-03535-72DOI1 aPEREA, G. N. R.  aElectrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillinbinvestigation of morphological features, antibiotic release and antimicrobial properties.h[electronic resource]  c2023  aPolymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides, the drug release kinetics study indicates that the nanocarrier system formed by PVP/CHI can release the antibiotics more slowly due to the chemical interactions between CHI and CLO compounds. Our results suggest that drug activity was preserved after encapsulation by electrospinning, and the diference in the inhibition zone diameter was corroborated by drug release mechanism experiments, indicating the system as a potential material to treat infections caused by S. aureus  aAntibiótico  aBovino  aDoença Animal  aTratamento  aAntibiotic release  aElectrospinning  aEletrofiação  aMastite  aSlow-release1 aPAVINATTO, A.1 aSCHNEIDER, R.1 aMUNK, M.1 aBRANDAO, H. de M.1 aCORREA, D. S.  tJournal of Polymer Researchgv. 30, 166, 2023.