02587naa a2200349 a 450000100080000000500110000800800410001902400440006010000220010424502500012626000090037630000100038552014930039565000170188865300230190565300140192865300150194265300120195765300230196965300150199265300140200770000220202170000140204370000160205770000180207370000250209170000160211670000200213270000260215270000130217877300460219121536812023-12-13       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.3390/nu150818312DOI1 aMISHIMA, M. D. V.  aEffects of Intra-Amniotic Administration of the Hydrolyzed Protein of Chia (Salvia hispanica L.) and Lacticaseibacillus paracasei on Intestinal Functionality, Morphology, and Bacterial Populations, In Vivo (Gallus gallus).h[electronic resource]  c2023  a17 p.  aAs a protein source, chia contains high concentrations of bioactive peptides. Probiotics support a healthy digestive tract and immune system. Our study evaluated the effects of the intra-amniotic administration of the hydrolyzed chia protein and the probiotic Lacticaseibacillus paracasei on intestinal bacterial populations, the intestinal barrier, the inflammatory response, and brush border membrane functionality in ovo (Gallus gallus). Fertile broiler (Gallus gallus) eggs (n = 9/group) were divided into 5 groups: (NI) non-injected; (H2O) 18 M? H2O; (CP) 10 mg/mL hydrolyzed chia protein; (CPP) 10 mg/mL hydrolyzed chia protein + 106 colony-forming unit (CFU) L. paracasei; (P) 106 CFU L. paracasei. The intra-amniotic administration was performed on day 17 of incubation. At hatching (day 21), the animals were euthanized, and the duodenum and cecum content were collected. The probiotic downregulated the gene expression of NF-??, increased Lactobacillus and E. coli, and reduced Clostridium populations. The hydrolyzed chia protein downregulated the gene expression of TNF-?, increased OCLN, MUC2, and aminopeptidase, reduced Bifidobacterium, and increased Lactobacillus. The three experimental groups improved in terms of intestinal morphology. The current results suggest that the intra-amniotic administration of the hydrolyzed chia protein or a probiotic promoted positive changes in terms of the intestinal inflammation, barrier, and morphology, improving intestinal health.  aInflammation  aBioactive peptides  aChia seed  aGut health  aIn vivo  aIntestinal barrier  aMicrobiota  aProbiotic1 aMARTINO, H. S. D.1 aKOLBA, N.1 aSHAH, D. D.1 aGRANCIERI, M.1 aSANTOS, K. M. O. dos1 aLIMA, J. P.1 aSILVA, B. P. DA1 aGONZALEZ DE MEJIA, E.1 aTAKO, E.  tNutrientsgv. 15, n. 8, p. 1831-15, 2023.