02727naa a2200553 a 450000100080000000500110000800800410001902400520006010000230011224501660013526000090030152011390031065000250144965000180147465000210149265000170151365000190153065000190154965000230156865000200159165000160161165000200162765000080164765000210165565300210167665300090169765300230170665300080172965300220173765300220175965300230178165300090180470000200181370000180183370000190185170000250187070000190189570000180191470000220193270000230195470000200197770000210199770000180201870000170203670000210205370000170207470000260209177300560211721526022023-11-27       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1038/s41598-023-31455-52DOI1 aSOUSA, A. L. M. de  aIn vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk.h[electronic resource]  c2023  aAbstract: This study aimed to evaluate, in vitro, the use of leaf extracts of Azadirachta indica (A. indica) and Melia azedarach (M. azedarach) as antivirals against caprine lentivirus (CLV) in colostrum and milk of goat nannies. These were collected from eight individuals and infected with the standard strain of CLV. Samples were then subdivided into aliquots and treated with 150 µg/mL of crude extract, and with ethyl acetate and methanol fractions for 30, 60, and 90 min. Next, somatic cells from colostrum and milk were co-cultured with cells from the ovine third eyelid. After this step, viral titers of the supernatants collected from treatments with greater efficacy in co-culture were assessed. The organic ethyl acetate fractions of both plants at 90 min possibly inhibited the viral activity of CLV by up to a thousandfold in colostrum. In milk, this inhibition was up to 800 times for the respective Meliaceae. In conclusion, the ethanolic fraction of ethyl acetate from both plants demonstrated efficacy against CLV in samples from colostrum and milk when subjected to treatment, which was more effective in colostrum.  aAntiviral properties  aGoat diseases  aMedicinal plants  aPhytotherapy  aPlant extracts  aSheep diseases  aAzadirachta Indica  aExtrato Vegetal  aFitoterapia  aMelia Azedarach  aNim  aPlanta Medicinal  aAntiviral plants  aCAEV  aCaprine Lentivirus  aCLV  aComposto bioativo  aEthanolic extract  aExtrato de plantas  aSRLV1 aPINHEIRO, R. R.1 aARAUJO, J. F.1 aPEIXOTO, R. M.1 aAZEVEDO, D. A. A. de1 aLIMA, A. M. C.1 aCANUTO, K. M.1 aRIBEIRO, P. R. V.1 aSOUZA, A. S. de Q.1 aSOUZA, S. C. R.1 aAMORIM, S. L. de1 aAMARAL, G. P.1 aSOUZA, V. de1 aMORAIS, S. M. de1 aANDRIOLI, A.1 aTEIXEIRA, M. F. da S.  tScientific Reportsgv. 13, article 4677, Mar. 2023.