01851naa a2200289 a 450000100080000000500110000800800410001902200140006002400540007410000240012824501280015226000090028052009910028965000110128065000170129165000180130865000390132665000100136565000160137565300150139165300270140665300230143370000190145670000280147570000200150377300380152321512742023-03-24       2023    bl uuuu     u00u1 u    #d  a2452-21987 ahttps://doi.org/10.1016/j.rhisph.2023.1006662DOI1 aMACHADO-ROSA, T. A.  aMicrotiter method for quantitative assay of IAA from fungal isolates, demonstrated with Trichoderma.h[electronic resource]  c2023  aA quantitative and accurate method is needed to estimate indole-3-acetic acid (IAA) production by Trichoderma to compare strains and allow the repeatability of experiments in plant-growth promotion studies. Suspensions of 1.0 × 106 conidia. mL-1 of 98 Brazilian strains were grown in potato + dextrose broth in the absence or presence of L-tryptophan (100 mg L-1) in 1 mL microtubes. After 12,000 rpm spinning for 15 min, 150 μL of the super-natant were added to 100 μL of Salkowski reagent with 30 min incubation at room temperature in the dark, with IAA yield spectrophotometrically estimated at 540 nm. Isolates BRM-29474 and BRM-29574 that outperformed in the IAA production increased tomato root and shoot mass and number of root tips in a greenhouse test, endorsing their potential for plant growth promotion. This adjustment allows a quantitative method to estimate an IAA production by Trichoderma spp., which is more informative than the standard qualitative method.  aAuxins  aPlant growth  aRoot exudates  aEstimulante de Crescimento Vegetal  aFungo  aTrichoderma  aBiocontrol  aPlant growth promotion  aStepwise screening1 aBARBOSA, E. T.1 aCÔRTES, M. V. de C. B.1 aLOBO JUNIOR, M.  tRhizospheregv. 25, 100666, 2023.