02579naa a2200253 a 450000100080000000500110000800800410001902400520006010000170011224502380012926000090036730000090037650000370038552017110042265000170213365000160215065000210216665300220218765300240220970000160223370000200224970000180226977300380228721509312023-03-27       2022    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s42535-022-00365-82DOI1 aCHANDOLA, V.  aAntioxidant potential and impact of different extraction solvents on the free, esterifed and insoluble-bound phenolics, flavonoid and tannin content of Trillium govanianum Wall ex D. Don, a rare Himalayan herb.h[electronic resource]  c2022  a8 p.  aPublished online: 07 April 2022.  aTrillium govanianum is a compelling medicinal herb that mainly occurs in the sub-alpine and alpine regions of the Himalayan states of India. It has acquired a threatened status due to large-scale illicit exploitation because of its effective medicinal properties. A limited number of studies on the antioxidant potential of T. govanianum along with its establishment as a source of new antioxidants led us to carry out this investigation. The study also investigated the efficacy of three different solvents on the quantitative extraction of bioactive compounds from the herb for its maximum utilization. For the study, rhizomes of T. govanianum were subjected to enzymatic and non-enzymatic assays. Analysis of enzymes catalase (CAT) and ascorbate peroxidase (AP) was carried out by simple phosphate buffer extraction method, phenolics and tannin determination by Folin-Ciocalteu method, flavonoid content by aluminium chloride colorimetric method and also total reducing and ferric reducing power assays were done. Results showed that rhizomes of T. govanianum exhibit fair CAT and AP activity. From the results it was also apparent that absolute methanol was the most feasible solvent among the all three used, to get the highest solid extractable yield (40.24%) as well as total phenolics and tannins (46.7 ± 0.15 and 0.74 ± 0.01 mg/gm of extract respectively) whereas acetone extract yielded more flavonoid content (16.5 ± 0.15 mg/gm of extract). FRAP activity and total reducing power of the methanol extract was also higher than the other two extracts. These findings reveal a promising potential of this herb as a source of antioxidants comprehending a need for its cultivation and conservation.  aAntioxidante  aFlavonóide  aPlanta Medicinal  aComposto bioativo  aTrillium govanianum1 aCHANDRA, S.1 aNAUTIYAL, A. R.1 aCONCENÇO, G.  tVegetosgv. 35, p. 953-960, 2022.