02119naa a2200385 a 450000100080000000500110000800800410001902400560006010000170011624501130013326000090024652010320025565000160128765000120130365000270131565300160134265300210135865300280137970000220140770000240142970000190145370000240147270000130149670000150150970000170152470000140154170000150155570000150157070000200158570000250160570000120163070000160164270000190165877300560167721486552023-02-13       2022    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.biortech.2022.1279992DOI1 aSERRA, L. A.  aScreening method for Enzyme-based liquefaction of corn stover pellets at high solids.h[electronic resource]  c2022  aLiquefaction of high solid loadings of unpretreated corn stover pellets has been demonstrated with rheology of the resulting slurries enabling mixing and movement within biorefinery bioreactors. However, some forms of pelleted stover do not readily liquefy, so it is important to screen out lots of unsuitable pellets before processing is initiated. This work reports a laboratory assay that rapidly assesses whether pellets have the potential for enzyme-based liquefaction at high solids loadings. Twenty-eight pelleted corn stover (harvested at the same time and location) were analyzed using 20 mL enzyme solutions (3 FPU cellulase/ g biomass) at 30 % w/v solids loading. Imaging together with measurement of reducing sugars were performed over 24-hours. Some samples formed concentrated slurries of 300 mg/mL (dry basis) in the small-scale assay, which was later confirmed in an agitated bioreactor. Also, the laboratory assay showed potential for optimizing enzyme formulations that could be employed for slurry formation.  aCorn stover  aEnzymes  aLignocellulosic wastes  aBiorefinery  aLaboratory assay  aLignocellulosic biomass1 aCRUZ, R. G. da S.1 aGUTIERREZ, D. M. R.1 aCRUZ, A. J. G.1 aCANIZARES, C. A. T.1 aCHEN, X.1 aMOSIER, N.1 aTHOMPSON, D.1 aASTON, J.1 aDOOLEY, J.1 aSHARMA, P.1 aDE MARCO, J. L.1 aALMEIDA, J. R. M. de1 aERK, K.1 aXIMENES, E.1 aLADISCH, M. R.  tBioresource Technologygv. 363, 127999, Nov., 2022.