02329naa a2200265 a 450000100080000000500110000800800410001902400520006010000230011224501330013526000090026852015270027765000240180465000150182865000110184365000170185465000100187165300250188165300200190670000170192670000180194370000220196170000210198377300590200421481042023-03-22 2022 bl uuuu u00u1 u #d7 ahttps://doi.org/10.1007/s13744-022-00996-12DOI1 aCARVALHO, K. S. de aToxicity of Bacillus thuringiensis strains to six lepidopteran pests of Brazilian agricultural landscape.h[electronic resource] c2022 aThe first step for biological insecticide production is the evaluating of the pathogenicity and strains virulence of entomopathogens against the target pests. Bacillus thuringiensis (Bt) is widespread in nature, lethal pathogen of a range of orders and the most widely used entomopathogenic biological control agent. Therefore, our objective was to study the susceptibility of six important lepidopteran pests in Brazilian agricultural production systems, Anticarsia gemmatalis (Hubner), Chrysodeixis includens (Walker) Helicoverpa armigera (Hubner), Spodoptera cosmioides (Walker), S. eridania (Cramer), and S. frugiperda (Smith), to Bt strains. In vitro bioassays were conducted with neonate larvae exposed to two Bt strains, 1680A and 775E, in seven concentrations of spores and protein crystals. After 7 days, the mortality was evaluated and indicated that lethal concentration (LC50) for 775E strain ranged from 7.72 x 10(4) to 2.45 x 10(6) spores mL(-1) and for 1608A strain from 5.63 x 10(3) to 1.21 x 10(6) spores mL(-1). The strain 1608A was more toxic for A. gemmatalis and H. armigera than 775E strain, and then was further evaluated in greenhouse bioassays with maize and soybean plants infested separately with target insects. The strain 1608A showed an efficient control for most insect species studied in the greenhouse bioassays, promoting considerable reduction in leaf injury and demonstrating a high potential in biological control of important lepidopteran species in crop production systems in Brazil. aControle Biológico aInseticida aInseto aLepidóptero aPraga aConcentração letal aSuscetibilidade1 aLEITE, N. A.1 aMENDES, S. M.1 aLANA, U. G. de P.1 aVALICENTE, F. H. tNeotropical Entomologygv. 51, n. 6, p. 869-876, 2022.