02097naa a2200217 a 450000100080000000500110000800800410001910000210006024500930008126000090017450000210018352015120020465000220171665000170173865000110175565000080176670000150177470000180178970000170180777300550182421478792022-10-31       2022    bl uuuu     u00u1 u    #d1 aZABALA-PARDO, D.  aRNAi as a tool for weed managementbchallenges and opportunities.h[electronic resource]  c2022  aSpecial issue 1.  aAbstract: RNA interference (RNAi) is a next-generation technology for weed management. Weeds would be sprayed with small RNAs (sRNAs) capable of inducing gene silencing (referred to as spray-induced gene silencing, SIGS) without involving the use of transgenes, reaching traditional targets in chemical control, targets that are not today more sensitive to herbicides and even new targets. Here we present the main challenges and opportunities for using SIGS as a practical tool for weed management. The development of SIGS in weed science has been slower compared to other crop protection areas such as entomology and plant pathology due to the difficulty of obtaining stable molecules that easily enter the plant, without off-target risks to crops, and that in small amounts guarantee systemic silencing with effectiveness. To overcome the challenges, it is necessary to achieve the synthesis of sRNAs on a large scale, making the field application practical and economical, develop formulations that protect sRNAs inside and outside the plant, and substantially increase the genomic and transcriptomic information available for weeds. Once these barriers have been overcome, SIGS technology could be similarly used in the field as herbicides are used today, spraying directly on the crop and selectively controlling weeds. This will provide a new tool for weed management, herbicide resistance management, and potentially exploration of new plant enzyme targets never before achieved by chemical control.  aCultivo Protegido  aErva Daninha  aManejo  aRNA1 aGAINES, T.1 aLAMEGO, F. P.1 aAVILA, L. A.  tAdvances in Weed Sciencegv. 40, e020220096, 2022.