02948naa a2200337 a 450000100080000000500110000800800410001902200140006002400540007410000230012824501730015126000090032452018930033365300240222665300260225065300250227665300110230170000250231270000180233770000190235570000190237470000300239370000200242370000180244370000220246170000170248370000220250070000190252270000190254177300500256021469702024-01-19       2022    bl uuuu     u00u1 u    #d  a0026-265X7 ahttps://doi.org/10.1016/j.microc.2022.1077452DOI1 aCÔNSOLO, N. R. B.  aUsing TD-NMR relaxometry to assess the effects of diet type and stocking rate on the incidence and degree of severity of myoparthies in broilers.h[electronic resource]  c2022  aThis study aimed to evaluate different feeding strategies and stocking rates (SR) on the occurrence and severity of broiler myopathies. Additionally, a second aim was to test the potential use of Time-Domain Nuclear Magnetic Resonance (TD-NMR) relaxometry for assessing white striping (WS) and wooden breast (WB) incidence and the degree of severity in broiler chicken breasts. Treatments were arranged in a 2 × 2 factorial design and consisted of two nutritional levels (regular and superior diets) and two different SR (high 47 kg/m2 or regular 59 kg/m2 ). Infrared thermography (IRT) images were recorded on three different days to determine the average, minimum and maximum temperatures. After 49 days of rearing, birds were slaughtered, and 75 breasts were weighed and classified according to the absence (normal breast, without the occurrence of myopathy ? NB) or occurrence and severity of WB and WS. The whole breasts were analysed by TD-NMR relaxometry using the Carr-PurcellMeiboom-Gill (CPMG) pulse sequence and Continuous Wave-Free Precession (CWFP-T1) pulse sequence. There was no effect of diet and diet*SR interaction on the weight of breast meat; however, chickens raised at the higher density presented a lower breast weight (P &lt 0.0001; 490 vs. 539 g). The incidence and severity of myopathies was not influenced by the treatments (P = 0.9467); the frequencies of WB and WS were similar in the different treatments. For CPMG and CWFP-T1, three and two relaxation populations were noted, respectively. The sPLS-DA model optimisation showed a significant separation between WS and NB in terms of occurrence and severity. In conclusion, changes in diet and SR did not mitigate the occurrence or severity of WS and WB. Additionally, TDNMR relaxometry showed to be a promising technology for evaluating and segregating breasts according to myopathy incidence and severity.  aEmerging technology  aMyopathies prediction  aT1 and T2 relaxation  aTD-NMR1 aBARBOSA, L. C. G. S.1 aMORAES, T. B.1 aBURAQUE, V. M.1 aCOLNAGO, L. A.1 aQUEIROZ JÚNIOR, L. H. K.1 aOLIVEIRA, V. M.1 aHANNAFORD, R.1 aARAÚJO, C. S. S.1 aROQUE, F. A.1 aGRANGHELLI, C. A.1 aFONSECA, A. C.1 aARAÚJO, L. F.  tMicrochemical Journalgv. 181, e107745, 2022.