01429naa a2200277 a 450000100080000000500110000800800410001910000250006024501020008526000090018752006400019665000200083665300220085665300190087865300130089770000220091070000180093270000150095070000210096570000160098670000200100270000180102270000180104070000180105877300750107621466022022-11-04       2022    bl uuuu     u00u1 u    #d1 aO'SULLIVAN, F. L. A.  aThe Piwil1 N domain is required for germ cell survival in Atlantic salmon.h[electronic resource]  c2022  aGenetic introgression of farmed salmon into wild populations can damage the genetic integrity of wild stocks and is therefore considered as an environmental threat. One possible solution is to induce sterility in farmed salmon. We have searched for proteins potentially essential for germline survival in Atlantic salmon. One of these is the argonaute protein Piwil1, known to be required for germ cell survival. To examine Piwil1 function in salmon, we induced indels in the N domain by CRISPR-Cas9. The encoded domain is present in all vertebrate Piwi proteins and has been linked to Tdrd1 protein interaction and PAZ lobe structure.  aSpermatogenesis  aArgonaute protein  aFish sterility  aGermline1 aSKAFTNESMO, K. O.1 aANDERSSON, E.1 aKLEPPE, L.1 aEDVARDSEN, R. B.1 aNORBERG, B.1 aFJELLDAL, P. G.1 aHANSEN, T. J.1 aSCHULZ, R. W.1 aWARGELIUS, A.  tFrontiers in Cell and Developmental Biologygv. 10, art. 977779, 2022.