02000naa a2200325 a 450000100080000000500110000800800410001902400570006010000190011724501310013626000090026752010040027665000210128065000210130165000100132265000130133265300200134565300190136565300190138465300190140365300170142270000240143970000230146370000200148670000220150670000240152870000240155270000230157677300750159921450252023-03-27       2022    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1590/1984-70332022v22n2a162DOI1 aRIBEIRO, M. R.  aAre stomatal area and stomatal density reliable traits for identification of doubled haploids in maize?h[electronic resource]  c2022  aTo meet the demands of the production chain, breeding programs need to reduce the time for selection and development of higher yielding maize genotypes. This involves application of new strategies, such as the use of the doubled haploid lines. We evaluated the use of stomatal area and density in distinguishing doubled haploids (DH) from false positive (FP) plants derived from nine tropical source populations. We selected four DH plants and two FP plants for identification of DH through leaf anatomy in the populations, in each group. Data on stomatal area and density were considered in a completely randomized model in a factorial scheme (populations and genotypes) and analyzed by restricted maximum likelihood. There were statistically significant effects of populations and genotypes for both traits. For stomatal density, the population by genotype interaction was not verified. Stomatal density proved to be efficient for distinguishing DH and FP for use in selection of doubled haploids.  aDoubled haploids  aAnatomia Vegetal  aMilho  aZea Mays  aAnatomia foliar  aDuplo haploide  aFalse positive  aFalso positivo  aLeaf anatomy1 aTRINDADE, R. dos S.1 aSOUZA, I. R. P. de1 aCARNEIRO, A. A.1 aAZEVEDO, T. C. de1 aGUIMARAES, L. J. M.1 aCHAVES, S. F. da S.1 aDIAS, L. A. dos S.  tCrop Breeding and Applied Biotechnologygv. 22, n. 2, e42222226, 2022.