02562naa a2200385 a 450000100080000000500110000800800410001902400550006010000210011524501720013626000090030852014320031765000170174965000130176665000160177965000160179565000100181165300240182165300200184565300140186565300160187965300230189565300260191865300190194465300210196365300160198465300260200065300160202670000220204270000140206470000110207870000190208970000130210877300550212121423782022-11-09       2022    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.foodres.2022.1112272DOI1 aVEREDIANO, T. A.  aBlack corn (Zea mays L.) soluble extract showed anti-inflammatory effects and improved the intestinal barrier integrity in vivo (Gallus gallus).h[electronic resource]  c2022  aBlack corn (Zea mays L.) is a pigmented type of this cereal whose color of the kernels is attributed to the presence of the anthocyanins. In this study, we assessed the black corn soluble extract (BCSE) effects on the intestinal functionality, morphology, and microbiota composition using an in vivo model (Gallus gallus) by an intraamniotic administration. The eggs were divided into four groups (n = 6?10): (1) No Injection; (2) 18 MΩ H2O/cm; (3) 5% (5 mg/mL) BCSE; (4) 15% (15 mg/mL) BCSE. The BCSE showed anti-inflammatory effects by down regulating the gene expression of tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL6), and the transcriptional nuclear factor kappa beta (NF-κB). Further, the BCSE increased the relative abundance of E. coli and Clostridium. 5% and 15% BCSE increased the hepatic glycogen and upregulated the gene expression of sodiumglucose transport protein (SGLT1). In the morphology, 5% and 15% BCSE increased the goblet cell (GC) number on the crypt, the GC size on the villi, Paneth cell number on the crypt, and the acid GC. Further, the BCSE strengthened the epithelial physical barrier through upregulating the intestinal biomarkers AMP- activated protein kinase (AMPK) and caudal-related homeobox transcriptional factor 2 (CDX2). The overall result suggests that the BCSE promotes intestinal anti-inflammatory effects as well as enhances the intestinal barrier function.  aAnthocyanins  aCyanidin  aPolyphenols  aAntocianina  aMilho  aBarreira intestinal  aCélula cálice  aCianidina  aGoblet cell  aIntestinal barrier  aIntestinal morphology  aIntra-amniotic  aIntra-amniótico  aMilho preto  aMorfologia intestinal  aPolifenóis1 aMARTINO, H. S. D.1 aKOLBA, N.1 aFU, I.1 aPAES, M. C. D.1 aTAKO, E.  tFood Research Internationalgv. 157, 111227, 2022.