02304naa a2200349 a 450000100080000000500110000800800410001902400550006010000210011524501750013626000090031150000180032052011930033865000120153165000290154365000200157265000220159265300280161465300250164265300250166765300290169270000190172170000250174070000220176570000200178770000190180770000200182670000210184670000140186770000210188177300520190221405822022-10-10       2022    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1080/10826068.2022.20339892DOI1 aSANTOS, A. DA S.  aProduction, concentration and partial characterization of an enzymatic extract produced by an Aspergillus niger mutant in solid state fermentation.h[electronic resource]  c2022  aEarly Access.  aAn enzymatic extract from Aspergillus niger 3T5B8 was produced by Solid State Fermentation (SSF) in aerated columns, using wheat bran as substrate. A combination of extracts produced using three different process conditions varying temperature, pH and aeration formed the final extract (Mixture). The Mixture was concentrated by an ultrafiltration process that partially purified and provided an efficient recovery of the enzymatic activities of xylanase (88.89%), polygalacturonase (89.3%), beta-glucosidase (93.15%), protease (98.68%) and carboxymethylcellulase (CMCase) (98.93%). SDS-PAGE analysis showed 15 visible protein bands in the crude and concentrated Mixture with molecular weights ranging from 15.1 to 104.6 kDa. Thin layer chromatography confirmed the effective action of beta-glucosidase and xylanase hydrolysis activities over cellobiose and xylan, respectively. A central composite design (CCD) with two variables and four replicates at the center points was used to determine the optimal temperature and pH for CMCase and beta-glucosidase. The optimal temperature was 78.9 degrees C and pH 3.8 for CMCase and 52.8 degrees C and pH 4.8 for beta-glucosidase, respectively.  aEnzymes  aSolid state fermentation  aUltrafiltration  aAspergillus Niger  aAspergillus niger 3T5B8  aCellulolytic enzymes  aEnzyme concentration  aPartial characterization1 aPEREIRA, G. M.1 aALMEIDA, A. M. M. DE1 aSANTOS, R. R. DOS1 aTERZI, S. da C.1 aPACHECO, T. F.1 aVERMELHO, A. B.1 aCABRAL, L. M. C.1 aCOURI, S.1 aDAMASO, M. C. T.  tPreparative Biochemistry & Biotechnology, 2022.