02271naa a2200349 a 450000100080000000500110000800800410001902400560006010000220011624501470013826000090028552011970029465000170149165000160150865000120152465300180153665300160155465300170157065300200158765300090160765300230161665300210163970000190166070000220167970000180170170000180171970000170173770000270175470000270178170000240180877300890183221389672022-02-14       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.ijbiomac.2020.11.0332DOI1 aLOURENÇON, T. V.  aAntioxidant, antibacterial and antitumoural activities of kraft lignin fromhardwood fractionated by acid precipitation.h[electronic resource]  c2021  aKraft lignin, so far useful for energy generation, has been gathering considerable attention as an alternative ma-terial to replace fossil-based resources mainly due to its high phenolic content. However, the wide molecularweight distribution and chemical composition heterogeneity led to the development of fractionation methods.Herein, to narrow such characteristics weusedeucalypt kraft lignin fractionated atpH's 9,7, 5 and 3 bysequentialacid precipitation. These lignin fractions werefirst characterised by simultaneous pyrolysis andtrimethylsilylation (SPyT) with N-Methyl-N-(trimethylsilyl) trifluoroacetamide with posterior tests of antioxi-dant, antibacterial, and antitumour activities. We observed higher ratio of syringyl/guaiacyl groups and increasein antioxidant activity in those fractions with lower molecular weight (precipitated at lower pH's). Fractions pre-cipitated at pH's 9 and 7 have shown an outstanding antibacterial activity againstfive bacteria. Moreover, frac-tions 7 and 5 presented at cytotoxicity tests higher ability to inhibit the growth of U87MG and T98G gliomacells, while only a slight inhibition of adult humanfibroblasts (non-tumour cells) was detected.  aAntioxidante  aBactericida  aLignina  aAntibacterial  aAntitumoral  aAntitumoural  aHardwood lignin  aIC50  aRadical scavenging  aTechnical lignin1 aLIMA, G. G. de1 aRIBEIRO, C. S. P.1 aHANSEL, F. A.1 aMACIEL, G. M.1 aSILVA, K. da1 aWINNISCHOFER, S. M. B.1 aBOLZON DE MUNIZ, G. I.1 aMAGALHAES, W. L. E.  tInternational Journal of Biological Macromoleculesgv. 166, p. 1535-1542, Jan. 2021.