02614naa a2200169 a 450000100080000000500110000800800410001902400520006010000220011224501570013426000090029152020080030065000270230870000290233570000250236477300550238921386862022-01-03       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s40858-021-00456-y2DOI1 aBATISTA, J. N. G.  aMolecular and phenotypic characterization of Xanthomonas campestris pv. campestris causing black rot in Brassica crops in Brazil.h[electronic resource]  c2021  aBlack rot caused by Xanthomonas campestris pv. campestris(Xcc) is one of the most important diseases affecting Brassica oleracea crops in Brazil. In the present study, 150 bacterial isolates from symptomatic leaf samples of broccoli, kale, Chinese cabbage, cauliflower, wild mustard, radish, and cabbage were identified and characterized phenotypically and genotypically. One isolate from a symptomatic tomato plant that grew spontaneously in a diseased cabbage field was also identified. Of these 151 isolates, 145 were confirmed as Xcc by multiplex PCR with Xcc and X. campestris pv. raphani(Xcr) specific primers and were pathogenic to cauliflower cv. Verona, including the isolate from tomato. The identity of six isolates that, although PCR positive for Xcc, were not pathogenic to cauliflower was determined by partial sequencing of the housekeeping rpoD gene, confirming that they were X. campestris. To investigate the ability of Xcc to infect tomato, 13 isolates were inoculated on tomato cv. Bonny Best, and six isolates, including the tomato isolate, induced chlorosis and small necrotic lesions on leaves and stems. Variability was assessed by BOX-PCR which resulted in 65 distinct haplotypes in the collection, with the largest number of haplotypes identified among broccoli isolates. In vitro copper and kasugamycin sensitivity tests showed that 143 isolates were resistant to 100 μg/mL of kasugamycin, and five isolates were resistant to 200 μg/mL of copper sulfate. From a subsample of 79 isolates, inoculated in eight differential genotypes, five races were detected (races 1, 3, 4, 6, and 9) with predominance of race 4 (54.43%, n = 43) and race 1 (32.91%, n = 26). The results provide tools for genetic breeding and the establishment of integrated black rot management programs for these important vegetables. Additionally, the newly found association between Xcc and tomato should be considered on the perspective of both brassica and tomato crop production in the country.  aXanthomonas Campestris1 aFERREIRA, M. A. da S. V.1 aQUEZADO-DUVAL, A. M.  tTropical Plant Pathologygv. 46, p. 684-701, 2021.