02702naa a2200325 a 450000100080000000500110000800800410001902400560006010000220011624501520013826000090029030000100029952017090030965000190201865000210203765300210205865300160207965300180209570000180211370000230213170000230215470000190217770000220219670000190221870000200223770000200225770000240227770000180230177300570231921371182021-12-15       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.jtherbio.2021.1028422DOI1 aGONÇALVES, A. A.  aScrotal thermoregulation and sequential sperm abnormalities in buffalo bulls (Bubalus bubalis) under short-term heat stress.h[electronic resource]  c2021  a10 p.  aHeat stress reduces the reproductive capacity of bulls raised in tropical climate. However, the reestablishment of scrotal thermoregulation and the dynamics of sperm defects emergence after stress are not completely known in buffaloes. Thus, the study aimed to evaluate the effect of short-term heat stress over scrotal thermoregulation and sperm attributes, relating them to spermatogenesis stages. Five buffalo bulls went through scrotal insulation during 48 h (from day 0 to day 2). Semen samples were collected every 7 days (from day - 7 to day 49) and progressive motility, viability, and sperm morphology. Heat stress significantly destabilized scrotal thermoregulation (P &lt 0.001). Scrotal temperature was from 4.2 to 6.3 C lower than the core body temperature, except on insulation days (P &lt 0.001), and returned to the basal condition five days after the removal of the stressing stimulus. More significant deleterious effects were observed in sperm morphology than in cell concentration, motility, and viability. The chronology of morphologic defects expression demonstrated tail defects (days 7?14), cytoplasmic droplets (days 14-28), and head defects (day 28), returning to pre-insulation condition 35 days after the thermal challenge. Thus, hyperthermia harmed more intensely spermatozoa in epididymal transit, elongated spermatids, and secondary spermatocytes. It is concluded that water buffalo bulls present a peculiar manifestation of sperm morphology after short-term stress, indicating an important difference related to the bovine species. Therefore, during the andrological evaluation of buffalo bulls, it is necessary to avoid the allometric extrapolation between these species.  aThermal stress  aThermoregulation  aSperm morphology  aSpermiogram  aWater buffalo1 aGARCIA, A. R.1 aROLIM FILHO, S. T.1 aSILVA, J. A. R. DA1 aMELO, D. N. DE1 aGUIMARÃES, T. C.1 aTAVARES, H. R.1 aSILVA, T. V. G.1 aSOUZA, E. B. DE1 aSANTOS, S. DO S. D.1 aOHASHI, O. M.  tJournal of Thermal Biologygv.96, feb. 2021, 102842.