01928naa a2200241 a 450000100080000000500110000800800410001902400550006010000190011524501580013426000090029252011280030165000260142965000230145565000290147865000180150765000120152570000190153770000220155670000200157870000200159877300680161821370282021-12-02       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1080/10826068.2021.19109592DOI1 aMARTINS, P. A.  aSolid-state fermentation production and characterization of an alkaline lipase from a newly isolated Burkholderia gladioli strain.h[electronic resource]  c2021  aThe newly isolated Burkholderia gladioli BRM58833 strain was shown to secrete an alkaline lipase highly active and stable in organic solvents. Lipase production was optimized through the cultivation of the strain by solid-state fermentation in wheat bran. The lipase extraction conditions were also optimized. T. Proteomic analysis revealed the optimized extract is composed of two esterases and three true lipases, showing a preference for long-chain substrates. The highest activity was obtained at 50 °C and pH 9. However, the extract maintained more than 50% of its maximum activity between pH 8.0 and 10.0 and throughout the whole temperature range evaluated (32?70 °C). The enzymes were inhibited by SDS, EDTA, ZnSO4 and FeCl3 and activated by FeSO4, MgCl2 and BaCl2. The lipases conserved their activity when incubated in solvents as acetonitrile, diethyl ether, n-heptane n-hexane, toluene, methanol and t-butanol. The resistance of these lipases to solvents and expressive thermostability when compared to other lipases, reveal their potential both in hydrolysis reactions and in synthesis of esters.  aBurkholderia gladioli  aProcess monitoring  aSolid state fermentation  aFermentação  aLípase1 aPACHECO, T. F.1 aCAMARGO, B. R. de1 aMARCO, J. L. de1 aSALUM, T. F. C.  tPreparative Biochemistry & Biotechnology, 3, p. 1-10, May 2021.