02103naa a2200277 a 450000100080000000500110000800800410001910000260006024501140008626000090020052013560020965000150156565000230158065000150160365000110161865000190162965000260164865300170167465300140169170000130170570000170171870000160173570000200175170000190177177300350179021367832021-11-29       2021    bl uuuu     u00u1 u    #d1 aSOUZA, T. C. G. D. de  aPorcine circovirus 3a field strains in free-livingwild boars in Paraná state, Brazil.h[electronic resource]  c2021  aAbstract: Porcine circovirus 3 (PCV-8208;3) was identified in domestic pigs worldwide. Although PCV-8208; 3 has also been detected in wild boars, information regarding its circulation in this free-8208;living animal species is scarce. To investigate PCV-8208;3 occurrence in free-8208;living wild boars in Brazil, 70 serum samples collected between January 2017 and June 2019 in Paraná state, Brazil were analyzed by PCR assay. Amplicons measuring 330 bp in length were amplified in seven (10.0%) of the serum samples and confirmed to be PCV3-8208;specific by nucleotide (nt) sequencing. As the amplified products from the serum samples yielded only intermediate levels of viral DNA, lung samples from the seven PCR-8208;positive wild boars were also evaluated by PCR. Of these samples, five lung samples were positive and provided high levels of viral DNA. The three lung samples that presented the highest levels of viral DNA were selected for amplification and sequencing of the whole PCV-8208;3 genome. The three full-8208;length sequences obtained were grouped in PCV-8208;3 clade "a", and the sequences exhibited 100% nucleotide similarity among them. The PCV-8208;3 field strains of this study showed nucleotide and amino acid similarities of 98.5-99.8% and 98.8-100%, respectively, with wholegenome PCV-8208;3 sequences from around the world.  aCircovirus  aPorcine circovirus  aSus scrofa  aJavali  aPorco Selvagem  aSus Scrofa Domesticus  aCircoviruses  aFeral pig1 aGAVA, D.1 aSCHAEFER, R.1 aLEME, R. A.1 aPORTO, G. da S.1 aALFIERI, A. A.  tAnimalsgv. 11, n. 1634, 2021.