02203naa a2200277 a 450000100080000000500110000800800410001902200140006002400520007410000230012624501480014926000090029752013220030665000140162865300260164265300150166865300220168365300210170570000260172670000230175270000160177570000180179170000200180970000170182977300790184621355342021-10-25       2021    bl uuuu     u00u1 u    #d  a0718-95087 ahttps://doi.org/10.1007/s42729-021-00561-62DOI1 aMONTEIRO, E. de C.  aStrategy for the sampling of sugarcane plants for the reliable quantifcation of N2 fixation Using 15N natural abundance.h[electronic resource]  c2021  aThe 15N natural abundance (δ15N) technique ofers good prospects for the quantifcation of the contribution of biological nitrogen fxation (BNF) to crop nutrition. The objectives of this study were to determine which plant parts best represented the whole plant with respect to δ15N and to quantify the BNF contribution to sugarcane. The experiment was carried out in pots of soil (100 kg pot−1) from the feld. The sugarcane varieties used were RB867515 and RB92579 with the reference plants Brachiaria decumbens, millet (Pennisetum americanum) and sorghum (Sorghum bicolor). The experimental design was randomised blocks with four replications. The treatments were inoculation of the sugarcane with or without diazotrophic bacteria and the diferent parts of the sugarcane plant (root, green leaf, senescent leaf and diferent stem sections). The δ15N of diferent cane parts and varieties ranged from+2.68 to+5.85? after 365 days, while the δ15N of reference plants were from+7.8 to+8.3?. The mean contribution of BNF to the sugarcane crop N supply was estimated to be 47% with no efect of variety or inoculation. The results indicate that the δ15N value of the entire shoot can be considered to represent the entire sugarcane plant, but no single organ could be relied on for this purpose  aSugarcane  a15N natural abundance  aN2 fxation  aNitrogen isotopes  aReference plants1 aSILVA, C. G. N. da S.1 aMARTINS, M. dos R.1 aREIS, V. M.1 aBODDEY, R. M.1 aALVES, B. J. R.1 aURQUIAGA, S.  tJournal of Soil Science and Plant Nutrition, publishing online 9 aug. 2021