01457naa a2200205 a 450000100080000000500110000800800410001902400540006010000170011424500910013126000090022252008680023165000170109970000170111670000180113370000160115170000160116770000190118377300490120221352032021-10-08       1982    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/0042-6822(82)90374-92DOI1 aELMERICH, C.  aCharacterization of a temperate bacteriophage for Azospirillum.h[electronic resource]  c1982  aA temperate bacteriophage, forming plaques on Azospirillum lipoferum strain Br17, was isolated from Brazilian soil samples. The phage morphology as revealed by electron microscopy showed an icosahedral head of 56 nm and a tail of 250 nm, to which six spikes are attached. The phage genome is double-stranded linear DNA of 36 kb with cohesive ends. Its host range is limited to strains of Brazilian origin, three being A. lipoferum and one A. brasilense. Spontaneous mutant phages having altered plaque morphology were isolated with a frequency of 10-4. Stable lysogens were obtained with all sensitive strains and shown to contain the prophage DNA maintained as a plasmid. Restriction analysis of the linear phage DNA and of the circular prophage provided proof of their identity and allowed identification of the restriction fragments carrying the cohesive ends.  aAzospirillum1 aQUIVIGER, B.1 aROSENBERG, C.1 aFRANCHE, C.1 aLAURENT, P.1 aDOBEREINER, J.  tVirologygv. 122, n. 1, p. 29-37, Oct. 1982.