02546naa a2200301 a 450000100080000000500110000800800410001902400520006010000170011224501270012926000090025650000380026552016280030365000180193165000230194965000120197265000240198465000110200865300210201965300180204065300240205870000200208270000130210270000220211570000210213770000330215877300530219121350762022-02-10       2022    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s13744-021-00917-82DOI1 aSTINGUEL, P.  aOptimization of in vivo production of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV).h[electronic resource]  c2022  aPublicado online em 29 set. 2021.  aInsect viruses have been used to protect crops and forests worldwide for decades. Among insect viruses, isolates of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) have proven potential for the control of Spodoptera frugiperda (J. E. Smith) (FAW) (Lepidoptera: Noctuidae), a pest of many economically essential crops across several continents. Mass production of SfMNPV depends on an in vivo system using host insect rearing. However, many factors can limit its production, including abiotic factors and host characteristics, such as the stage of development and an antagonist intraspecifc interaction. Thus, to improve in vivo production, we verifed the most suitable larval age to inoculate the virus and the infuence of incubation temperature on viral production. Subsequently, cannibal behavior was verifed in FAW larvae reared at diferent densities, while reproducing the conditions of the best treatments. The highest viral yield occurred when FAW larvae were inoculated at 10 and 8 days old and incubated at 22 °C and 25 °C, respectively. Nonetheless, survival (lethal period in days) and cannibal behavior were positively infuenced by larval development, which potentially increases the load of contamination and requires larval individualization for these production conditions. In contrast, 4-day-old larvae, which were inoculated and incubated at 31 °C, also demonstrated high viral production, with lower rates of cannibalism and death on the same day, thereby showing potential. The information presented in this study is useful for the optimization of the in vivo production systems of SfMNPV.  aBaculoviridae  aBiological control  aViruses  aControle Biológico  aVírus  aEntomopathogenic  aFall armyworm  aLagarta do cartucho1 aPAIVA, C. A. C.1 aZUIM, V.1 aAZEVEDO, A. C. T.1 aVALICENTE, F. H.1 aSANTOS JÚNIOR, H. J. G. dos  tNeotropical Entomologygv. 51, p. 122-132, 2022.