03484naa a2200289 a 450000100080000000500110000800800410001910000260006024500950008626000090018152026400019065000110283065000260284165000120286765000220287965000340290165000180293565000220295365000110297565000180298670000220300470000300302670000260305670000210308270000210310377300700312421344982021-09-16       2021    bl uuuu     u00u1 u    #d1 aMEISSNER FILHO, P. E.  aResistance of transgenic papaya trees to papaya ringspot in Brazil.h[electronic resource]  c2021  aABSTRACT - The objective of this work was to evaluate the resistance of transgenic papaya populations (PTPs) to Papaya ringspot virus-P (PRSV-P). 'Sunrise Solo' transgenic papaya plants were produced with the gene of the PRSV-P protein coat, and PRSV was mechanically inoculated in plants in greenhouse conditions. The presence of the CP/PRSV gene and homozygosis were evaluated by polymerase chain reaction (PCR). Selected plants and the 'Sunrise Solo' control were transplanted to the field for agronomic evaluations. The plants evaluated in greenhouse conditions showed resistance between 96.3 and 5.8%, without variation of symptoms. The PTPs 1/6, 1/7, 1/9, 1/10, 1/15, 2/38, 2/41, 2/56, 2/65, 3/27, 3/46, 3/48, 4/9, 4/27, 8/4, 8/23, 8/33, 18/3, 18/4, 18/8, 18/22, 18/27, 28/97, 28/104, and 28/110 showed no symptoms, they were ELISA negative, and most of them contained the CP and NPT II genes. PTPs 1/6 and 3/46 had the CP gene in homozygosis and in double insertion. PTPs 1/6/20, 1/6/59, 1/6/64, 1/6/90, 3/46/44, 3/46/52, and 18/27/97 had a well-formed fruit cluster, piriform fruit weighing approximately 500 grams, orange pulp, and less than 10% carpelloidy. PTPs 1/6/59 and 3/46/52 show resistance to PRSV, good agronomic characteristics, and the CP gene in homozygosis. RESUMO - O objetivo deste trabalho foi avaliar a resistência de populações transgênicas de mamoeiro (PTPs) ao vírus da mancha anelar do mamoeiro [Papaya ringspot virus-P (PRSV-P)]. Plantas transgênicas 'Sunrise Solo' foram produzidas com o gene da proteína da capa viral PRSV-P, e o PRSV foi inoculado mecanicamente nas plantas em casa de vegetação. A presença do gene CP/PRSV e a homozigose foram avaliadas por reação em cadeia da polimerase. As plantas selecionadas e o controle 'Sunrise Solo' foram transplantados para o campo, para avaliações agronômicas. As plantas avaliadas em casa de vegetação apresentaram resistência entre 96,3 e 5,8%, sem variação nos sintomas. As PTPs 1/6, 1/7, 1/9, 1/10, 1/15, 2/38, 2/41, 2/56, 2/65, 3/27, 3/46, 3/48, 4/9, 4/27, 8/4, 23/8, 33/33, 18/3, 18/4, 18/8, 18/22, 18/27, 28/97, 28/104 e 28/110 não apresentaram sintomas, tiveram resultado negativo ao teste ELISA, e a maioria delas continha os genes CP e NPT II. As PTPs 1/6 e 3/46 apresentaram gene CP em homozigose e em dupla inserção. As PTPS 1/6/20, 1/6/59, 1/6/64, 1/6/90, 3/46/44, 3/46/52 e 18/27/97 apresentaram cacho de frutos bem formado, frutos piriformes com 500 g, polpa laranja e menos de 10% de carpeloidia. As PTPS 1/6/59 e 3/46/52 apresentam resistência ao PRSV, boas características agronômicas e o gene CP em homozigose.  aCarica  aPapaya ringspot virus  aPapayas  aTransgenic plants  aCaracterísticas Agronômicas  aCarica Papaya  aDoença de Planta  aMamão  aMancha Anelar1 aVILARINHOS, A. D.1 aOLIVEIRA, V. J. dos S. de1 aSILVA, D. de C. S. da1 aSANTOS, V. da S.1 aDANTAS, J. L. L.  tPesquisa Agropecuária Brasileira, Brasíliagv.56, e01954, 2021.