02271naa a2200277 a 450000100080000000500110000800800410001902400460006010000170010624501330012326000090025650000220026552014200028765000200170765000180172765000120174565000200175765000100177765000300178770000280181770000180184570000220186370000190188570000150190477300740191921319382021-06-15       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1155/2021/55726302DOI1 aNIGRA, A. D.  aAntitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines.h[electronic resource]  c2021  aResearch Article.  aCoffee consumption is believed to have chemopreventive and chemotherapeutic effects and to contribute to preventing thedevelopment and progression of cancer. However, there is still controversy around these claims. As indicated in our previousworks, diet can influence the risk of breast cancer. Intake of coffee is hypothesized to reduce this risk, but current scientificevidence is not conclusive. This work is aimed at studying the effects of Robusta coffee bean extract on cell viability,proliferation, and apoptosis of different human cancers, especially breast cancer cell lines. To this end, cell viability wasevaluated by Alamar Blue in 2D and 3D models, the cell cycle by PI, apoptosis by annexin V, mitochondrial morphology, andfunctionality by mitoTracker, and colony formation capacity by the clonogenic assay. Green and dark coffee extract significantlyreduced viability in human breast, colorectal, brain, and bone cancer cells. Coffee anticancer activity was clearly evidenced inMDA-MB-231 (ER-) and MCF-7 (ER+) breast cancer cells but not in the normal breast cell line. In addition, coffee extractinduces an increase S phase and a decrease G2/M population in breast cancer cells, affected the mitochondrial morphology, andtriggered apoptosis. MDA-MB-231 breast cancer cells lost their clonogenic capacity after treatment. The antitumor activity wasdemonstrated in both 2D and 3D culture cell models  aFood technology  aCafé Robusta  aCâncer  aExtrato Vegetal  aGrão  aProduto de Origem Vegetal1 aGUIMAREAES, D. de A. B.1 aPRUCCA, C. G.1 aFREITAS-SILVA, O.1 aTEODORO, A. J.1 aGIL, G. A.  tOxidative Medicine and Cellular Longevity, 2021, ID 5572630, p. 1-16.