02426naa a2200445 a 450000100080000000500110000800800410001902400430006010000160010324501740011926000090029352011110030265000110141365000190142465000200144365000210146365000120148465300240149665300160152065300260153670000200156270000200158270000190160270000190162170000160164070000210165670000180167770000190169570000260171470000200174070000180176070000180177870000210179670000230181770000240184070000230186470000230188770000210191077300490193121307672021-03-17       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1111/tpj.150462DOI1 aMOTA, T. R.  aSuppression of a BAHD acyltransferase decreases p-coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis.h[electronic resource]  c2021  aAbstract: Grass cell walls have hydroxycinnamic acids attached to arabinosyl residues of arabinoxylan (AX), and certain BAHD acyltransferases are involved in their addition. In this study, we characterized one of these BAHD genes in the cell wall of the model grass Setaria viridis. RNAi silenced lines of S. viridis (SvBAHD05) presented a decrease of up to 42% of ester-linked p-coumarate (pCA) and 50% of pCA-arabinofuranosyl, across three generations. Biomass from SvBAHD05 silenced plants exhibited up to 32% increase in biomass saccharification after acid pre-treatment, with no change in total lignin. Molecular dynamics simulations suggested that SvBAHD05 is a p-coumaroyl coenzyme A transferase (PAT) mainly involved in the addition of pCA to the arabinofuranosyl residues of AX in Setaria. Thus, our results provide evidence of p-coumaroylation of AX promoted by SvBAHD05 acyltransferase in the cell wall of the model grass S. viridis. Furthermore, SvBAHD05 is a promising biotechnological target to engineer crops for improved biomass digestibility for biofuels, biorefineries and animal feeding.  aLignin  aLignocellulose  aP-coumaric acid  aSaccharification  aLignina  aCell wall acylation  aGrass xylan  aHydroxycinnamic acids1 aSOUZA, W. R. DE1 aOLIVEIRA, D. M.1 aMARTINS, P. K.1 aSAMPAIO, B. L.1 aVINECKY, F.1 aRIBEIRO, ANA. P.1 aDUARTE, K. E.1 aPACHECO, T. F.1 aMONTEIRO, N. DE K. V.1 aCAMPANHA, R. B.1 aMARCHIOSI, R.1 aVIEIRA, D. S.1 aKOBAYASHI, A. K.1 aOLIVEIRA, P. A. de1 aFERRARESE-FILHO, O.1 aMITCHELL, R. A. C.1 aMOLINARI, H. B. C.1 aSANTOS, W. D. D.  tThe Plant Journalgn. 105, p. 136-150, 2021.