02499naa a2200229 a 450000100080000000500110000800800410001902400510006010000220011124501650013326000090029852017160030765300330202365300260205670000240208270000250210670000230213170000170215470000170217170000190218877300620220721306092021-03-11       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s12223-020-00844-2DOI1 aRODRIGUEZ YON, Y.  aDevelopment of a taxon discriminating molecular marker to trace and quantify a mycorrhizal inoculum in roots and soils of agroecosystems.h[electronic resource]  c2021  aCrop inoculation with Glomus cubense isolate (INCAM-4, DAOM-241198) promotes yield in banana, cassava, forages, and others. Yield improvements range from 20 to 80% depending on crops, nutrient supply, and edaphoclimatic conditions. However, it is difcult to connect yield efects with G. cubense abundance in roots due to the lack of an adequate methodology to trace this taxon in the feld. It is necessary to establish an accurate evaluation framework of its contribution to root colonization separated from native arbuscular mycorrhizal fungi (AMF). A taxon-discriminating primer set was designed based on the ITS nrDNA marker and two molecular approaches were optimized and validated (endpoint PCR and quantitative real-time PCR) to trace and quantify the G. cubense isolate in root and soil samples under greenhouse and environmental conditions. The detection limit and specifcity assays were performed by both approaches. Diferent 18 AMF taxa were used for endpoint PCR specifcity assay, showing that primers specifcally amplifed the INCAM-4 isolate yielding a 370 bp-PCR product. In the greenhouse, Urochloa brizantha plants inoculated with three isolates (Rhizophagus irregularis, R. clarus, and G. cubense) and environmental root and soil samples were successfully traced and quantifed by qPCR. The AMF root colonization reached 41?70% and the spore number 4?128 per g of soil. This study demonstrates for the frst time the feasibility to trace and quantify the G. cubense isolate using a taxon-discriminating ITS marker in roots and soils. The validated approaches reveal their potential to be used for the quality control of other mycorrhizal inoculants and their relative quantifcation in agroecosyst.  aArbuscular mycorrhizal fungi  aMycorrhizal inoculant1 aMAISTRO PATREZE, C.1 aSAGGIN JUNIOR, O. J.1 aANTONIO RIVERA, R.1 aQUINONES, M.1 aHAESAERT, G.1 aVAN TUINEN, D.  tFolia Microbiologica, published online: 03 february 2021.