02682naa a2200289 a 450000100080000000500110000800800410001902400340006010000150009424501990010926000090030850004350031752013360075265000200208865000270210865300280213565300260216365300290218965300190221865300280223770000170226570000210228270000150230370000150231870000140233377300450234721254282020-11-18       2020    bl uuuu     u00u1 u    #d7 adoi:10.3390/foods91014152DOI1 aOZORIO, L.  aThe Influence of Peptidases in Intestinal Brush Border Membranes on the Absorption of Oligopeptides from Whey Protein HydrolysatebAn Ex Vivo Study Usingan Ussing Chamber.h[electronic resource]  c2020  aFinanciamento: Fundação Carlos Chagas Filho; Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ); E-26/110.698/201; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq);  Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA); Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE).  aFor many years, it was believed that only amino acids, dipeptides, and tripeptides couldbe absorbed and thus reach the bloodstream. Nowadays, the bioavailability of oligopeptides is alsoconsidered possible, leading to new research. This pilot study investigates the activity of brushborder enzymes on undigested whey protein hydrolysate (WPH) and on simulated intestinal digested(ID) whey hydrolysate and the subsequent absorption of resultant peptides through the proximaljejunum of a 7-week old piglet setup in an Ussing chamber model. Amongst all samples taken,884 oligopeptides were identified. The brush border peptidase activity was intense in the first 10 minof the experiment, producing several new peptides in the apical compartment. With respect to the IDsubstrate, 286 peptides were detected in the basolateral compartment after 120 min of enzyme activity,originating from?-lactoglobulin (60%) and?-casein (20%). Nevertheless, only 0.6 to 3.35% of anyspecific peptide could pass through the epithelial barrier and thus reach the basolateral compartment.This study demonstrates transepithelial jejunum absorption of whey oligopeptides in an ex vivomodel. It also confirmed the proteolytic activity of brush border enzymes on these oligopeptides,giving birth to a myriad of new bioactive peptides available for absorption.  aFood technology  aTecnologia de Alimento  aBrush border peptidases  aIntestinal permeation  aOligopeptides absorption  aUssing chamber  aWhey protein hydrolysat1 aSILVA, C. M.1 aCABRAL, L. M. C.1 aJARDIN, J.1 aBOUDRY, G.1 aDUPON, D.  tFoodsgv. 9, n. 10, 1415, 2020. p. 1-15.