02539naa a2200325 a 450000100080000000500110000800800410001902200140006002400520007410000190012624501850014526000090033052015310033965000100187065000160188065000220189665000150191865000120193365000290194565000100197465000290198465000230201365000150203670000180205170000200206970000260208970000190211570000190213477300600215321242292021-01-12       2020    bl uuuu     u00u1 u    #d  a1573-50367 ahttps://doi.org/10.1007/s11104-020-04654-62DOI1 aPACHECO, R. S.  aDifferences in contribution of biological nitrogen fixation to yield performance of common bean cultivars as assessed by the 15N natural abundance technique.h[electronic resource]  c2020  aAims Identification of variability in biological N2 fixation (BNF) contribution among common bean (Phaseolus vulgaris L.) cultivars under field conditions requires a reliable methodology. This study aimed to assess different common bean cultivars for plant growth and grain yield and to quantify the BNF contribution to the crop using the 15N natural abundance technique. Methods Two field experiments were conducted in an Oxisol in Brazil over two consecutive years, with eight common bean cultivars inoculated with rhizobium or fertilized with mineral N. Plants were analysed at mid-pod filling stage (two weeks after full-flowering) and at grain maturity. BNF was estimated by the 15N natural abundance technique. Results Average grain yields were 1614 or 2942 kg ha-1 in Experiment I, and 3284 or 3919 kg ha-1 in Experiment II, with rhizobium inoculation or mineral N, respectively. The average contributions of N derived from atmosphere were 14 and 26%, and amounts of N2-fixed were 7 and 22 kg N ha-1, in bean plants at mid-pod filling, respectively, in Experiment I and Experiment II. Conclusions The contributions of BNF increase when common bean crop reached its optimum yield potential even though soil N was the most important source for the plants. There is good relationship between delta15N values of grains and shoots, provided cultivars are of similar growth habit. The 15N natural abundance technique allowed identifying cultivars with relatively high BNF capability for commercial crop and breeding purposes.  abeans  agrain yield  anitrogen fixation  aAbsorção  aFeijão  aFixação de Nitrogênio  aGrão  aNitrogênio Atmosférico  aPhaseolus Vulgaris  aRendimento1 aBODDEY, R. M.1 aALVES, B. J. R.1 aFERREIRA, E. P. de B.1 aSTRALIOTTO, R.1 aARAÚJO, A. P.  tPlant and Soilgv. 454, n. 1/2, p. 327-341, Sept. 2020.