03030naa a2200337 a 450000100080000000500110000800800410001902400520006010000200011224502260013226000090035852018910036765000190225865000130227765000140229065000120230465000210231665300260233765300400236365300150240365300150241870000190243370000250245270000250247770000220250270000220252470000250254670000150257170000280258677300780261421234032020-06-22       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s42729-019-00128-62DOI1 aOLIVEIRA, G. S.  aAre cowpea-nodulating Bradyrhizobial communities influenced by bibochar amendments in soils? genetic diversity and symbiotic effectiveness assessment of two agricultural soils of Brazilian drylands.h[electronic resource]  c2020  aThe objective of this study was to evaluate the influence of biochar amendment on the bacterial genetic diversity and symbiosis in cowpea-nodulating rhizobial communities in two agricultural soils of Brazilian drylands. Vertisol and ultisol surface layer soil samples were collected in the Brazilian semiarid region. The soils were mixed with biochar and cowpea seeds (BRS Marataoã) and sown in pots. Forty-five days after plant emergence, the nodule bacteria were isolated. The genetic variability of the nodule bacteria was evaluated by means of restriction fragment length polymorphism of 16S-23S rRNA intergenic spacer region (IGS-RFLP), and those that shared identical IGS-RFLP profiles were fingerprinted by BOX-PCR. By selecting of representative isolates in the genetic clusters, 27 bacteria were identified by 16S-23S IGS sequencing and symbiotically assessed. The IGS-RFLP dendrogram of the 73 isolates had 20 clusters; the multivariate correspondence analysis grouped both Vertisol treatments, and the Ultisol treatments were placed far away in the biplot. Ultisol showed higher bradyrhizobial diversity than the Vertisol. The fingerprinting indicated the presence of only three clonal colonies, showing high intraspecific diversity. The 16S-23S IGS sequences indicated the prevalence of bacteria related to Bradyrhizobium guangxiense in the Vertisol and Bradyrhizobium zhanjiangense in the Ultisol, in addition to the presence of three putative Microvirga spp. in the Ultisol. The symbiotic efficiency of 22 out of 27 bacteria was comparable with that of the inoculant strain Bradyrhizobium yuanmingense BR 3267. The genetic diversity of the cowpea-nodulating bradyrhizobia was not primarily affected by the biochar application in two agricultural soils of Brazilian drylands. Both soils harbored symbiotically efficient bacteria, without the influence of biochar amendment  aBradyrhizobium  aAmendoim  aBactéria  aFeijão  aRhizobium Cowpea  aDiversidade genética  aFixação biológica de nitrogênio  aMicrovirga  aSemiárido1 aSENA, P. T. S.1 aNASCIMENTO, T. R. do1 aFERREIRA NETO, R. A.1 aPEREIRA, J. R. C.1 aMARTINS, L. M. V.1 aFREITAS, A. D. S. de1 aSIGNOR, D.1 aFERNANDES JUNIOR, P. I.  tJournal of Soil Science and Plant Nutritiongv.20, n.2, p. 439-449, 2020.