01689naa a2200217 a 450000100080000000500110000800800410001910000270006024501280008726000090021552010460022465000110127070000270128170000230130870000200133170000260135170000180137770000200139570000200141577300360143521228082021-11-29       2020    bl uuuu     u00u1 u    #d1 aRAMOS-GONZÁLEZ, P. L.  aFirst genome sequence of an isolate of hibiscus chlorotic ringspotvirus from the Western hemisphere.h[electronic resource]  c2020  aFor the first time, the near-complete genome sequence of the betacarmovirus, hibiscus chlorotic ringspot virus (HCRSV) isolated from the Americas is disclosed. High throughput sequencing of the total RNA extract from a Hibiscus-rosa sinensis (L.) plant collected in São Paulo state, Brazil, revealed the genome sequence of HCRSV isolate SBO1, which is 3945 nucleotides long and shows 93.1% nucleotide sequence identity with HCRSV_Singapore (X86448), considered the type member of the species. These two viruses display a similar genomic organization and potentially encode seven open reading frames (ORFs). In addition, a phylogenetic analysis based on a fragment with 557 nts of p38, the coat protein gene, from a cohort of 14 samples collected in Brazil revealed a no clearly defined segregation of South American isolates from those previously detected in geographical areas outside this continent. The practical consequences of the use of p38 ORF-derived amplicons for detection and variability studies of HCRSV are concisely discussed.  aVírus1 aCOSTA-RODRIGUES, M. da1 aPOTSCLAM-BARRO, M.1 aCHABI-JESUS, C.1 aBANGUELA-CASTILLO, A.1 aHARAKAVA, RI.1 aKITAJIMA, E. W.1 aASTUA, J. de F.  tTropical Plant Pathology, 2020.