03355naa a2200433 a 450000100080000000500110000800800410001902400620006010000210012224501450014326000090028852021020029765000200239965000180241965000090243765000100244665000200245665000140247665000180249065000250250865000110253365000100254465000110255465300210256565300250258665300200261165300330263170000180266470000210268270000270270370000210273070000170275170000200276870000200278870000190280870000220282770000200284977300520286921225042020-05-22       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.theriogenology.2020.05.0042DOI1 aSANTOS, J. D. R.  aTranscervical vs. laparotomy embryo collection in ewesbThe effectiveness and welfare implications of each technique.h[electronic resource]  c2020  aAbstract: This study assessed animal welfare in ewes subjected to transcervical (TC) or laparotomy (LP) embryo collection, and the efficiency of these two techniques. Santa Inês ewes (n = 57) received a protocol for estrus synchronization and superovulation. Cervical dilation protocol was initiated 12 h before embryo collection in all ewes. Depending on the success of cervical passage, the embryos were collected from ewes by either TC or LP. Records were made of physiological (rectal temperature (RT) and heart rate (HR)), endocrine (cortisol concentration), biochemical (glycaemia, total proteins, globulin and albumin concentrations), and behavioral variables. Data were recorded before fasting (BF) and sedation (BS), during (DC) and immediately after embryo collection (IAC), and 1 h (1hAC), 3 h (3hAC), 6 h (6hAC), 12 h (12hAC), 24 h (24hAC), and 48 h (48hAC) after embryo collection. The LP and TC procedures were applied to 22 and 35 ewes (with 100.0% and 94.3% of procedures being successful, respectively). The use of LP took longer than TC (P = 0.007) but was less effective in the recovery of uterine fluid and structures (P = 0.0002 and P = 0.0180, respectively), with no difference in the number of viable embryos recovered per animal. The TC procedure induced a greater RT at DC (P = 0.002) and IAC moments (P &lt 0.0001). The heart rate was greater in TC than LP in IAC (P = 0.036). On the other hand, HR was greater with LP at 12hAC (P = 0.033) and 24hAC (P = 0.002). There was no interaction between the procedures and time on total proteins, albumin, or globulin concentrations. The TC procedure induced greater glycaemia than LP in IAC (P &lt 0.0001). LP induced greater serum cortisol concentration than TC at DC, IAC, 1hAC (P = 0.0004; P = 0.0006; P = 0.036, respectively), even though it was greater in the TC than the LP procedure at 3hAC (P = 0.008). In conclusion, the TC embryo collection was more effective than the traditional LP procedure. Although both embryo collection procedures affected ewes? welfare, the TC procedure is probably less stressor than the LP.  aAnimal behavior  aAnimal stress  aEwes  aSheep  aSmall ruminants  aBem-Estar  aBiotecnologia  aComportamento Animal  aOvelha  aOvino  aStress  aAnimal behaviour  aAnimal biotechnology  aEmbryo recovery  aReproductive biotechnologies1 aUNGERFELD, R.1 aBALARO, M. F. A.1 aSOUZA-FABJAN, J. M. G.1 aCOSENTINO, I. O.1 aBRAIR, V. L.1 aSOUZA, C. V. de1 aPINTO, P. H. N.1 aBADE, A. L. C.1 aFONSECA, J. F. da1 aBRANDÃO, F. Z.  tTheriogenologygv. 153, p. 112-121, Sept. 2020.