02669naa a2200373 a 450000100080000000500110000800800410001902400590006010000230011924501570014226000090029952014650030865000240177365000370179765000200183465000270185465000100188165000190189165000140191065000340192465000100195865000240196865000200199265000310201265300140204365300370205770000200209470000270211470000220214170000220216370000200218570000220220577300680222721218732020-04-28       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.anireprosci.2020.1064692DOI1 aOLIVEIRA, M. E. F.  aRepeated trans-cervical embryo recoveries in Santa inês ewes subjected to short- or long-term superovulatory treatment regimens.h[electronic resource]  c2020  aAbstract: Outcomes of short- (6.5 days) and long-term (14.5 days) estrous synchronization for 6.5 d (G-6.5d) or 14.5 d (G-14.5d) and followed by the 4-day or 3-day declining-dose follicle-stimulating hormone superovulatory regimen, respectively, were compared using 16 estrous-cycling Santa Inês ewes. Non-surgical embryo recovery (NSER) procedures were performed 60 d apart starting 6 or 7 d after the onset of estrus; an i.m. injection of estradiol benzoate and of D-cloprostenol at 16 h was followed by an i.v. oxytocin injection administered 20 min before NSER. There was a longer (P&lt0.05) period before estrous onset in ewes during the second (September) compared with the first study replicate (July) by approximately 14 h. The NSER could be performed in 11 of 15 ewes that were in estrus, with an average of three viable-embryos/donor and the mean duration of the procedure being 29 min. There were no differences in superovulatory responses between the two groups of ewes, but there were only degenerated embryos in ewes of the G-6.5d group. In summary: i. the duration of progestin-priming and of multiple-dose pFSH treatment had a limited effect on superovulatory responses in estrous-cycling Santa Inês ewes; ii. NSER is a safe and repeatable method of embryo collection in ewes subsequent to superovulation; and iii. duration of the superovulatory treatment regimen may alter the effects of endogenous steroids on oocyte/embryo quality in ewes.  aAnimal reproduction  aBreeding and Genetic Improvement  aEmbryo transfer  aEstrus synchronization  aSheep  aSuperovulation  aEstradiol  aMelhoramento Genético Animal  aOvino  aReprodução Animal  aSuperovulação  aTransferência de Embrião  aProgestin  aTrans -cervical uterine flushing1 aZAMBRINI, F. N.1 aSOUZA-FABJAN, J. M. G.1 aBARTLEWSKI, P. M.1 aGUIMARÃES, J. D.1 aBRANDÃO, F. Z.1 aFONSECA, J. F. da  tAnimal Reproduction Sciencegv. 2017, article 106469 Jun. 2020.