03037naa a2200337 a 450000100080000000500110000800800410001902400540006010000200011424500990013426000090023330000090024252020490025165000230230065000220232365000180234565000190236365000170238265000130239965000140241265000140242665300290244065300360246965300160250565300240252170000290254570000200257470000210259470000200261577300640263521216362023-02-07 2020 bl uuuu u00u1 u #d7 ahttps://doi.org/10.1016/j.vetpar.2020.1090362DOI1 aFERREIRA, L. L. aAttract or repel Amblyomma sculptum ticksbscreening of semiochemicals.h[electronic resource] c2020 a6 p. aAbstract: Amblyomma sculptum is a tick of medical-veterinary importance. Areas with high infestations need to be monitored, and parasitizedhosts treated accordingly. Carbon dioxide (CO2) traps and acaricides are commonly deployed as control measures, although with some disadvantages suchas high costs, challenging logistics and vertebrate intoxication. Semiochemicals have the potential to improve tick attraction to traps and monitoring devicesand alleviate the burden of A. sculptum infestations. Four concentrations (10, 5, 2.5 and 1.25 %) of 13 semiochemical candidates (CO2 only at 5 % as thestandard, benzaldehyde, benzoic acid, salicylic acid, 2,6 diclorophenol, R-limonene, S-limonene, methyl salicylate, 1-octen-3-ol, acetone, ammoniumhydroxide, isobutyric acid and lactic acid) were tested on unfed A. sculptum nymphs and adults using a Y-tube olfactometer to evaluated repellence andattraction behaviors. All stages tested were attracted to CO2, whereas nymphs were repelled by benzaldehyde and R-limonene, both at 10 %, and isobutyricacid at 5 and 10 %. Nymphs were attracted by methyl salicylate, benzoic acid and salicylic acid, all at 1.25 %, and by ammonium hydroxide at 2.5 %. Maleswere attracted by benzoic acid at 2.5 %, while females were repelled by benzaldehyde at 5 %. Mixtures with the attractive compounds achieved noattraction response. The compounds that caused attractiveness in the olfactometer assay (CO2, methyl salicylate, benzoic acid, salicylic acid and ammoniumhydroxide) were placed randomly in traps in a grassland plot naturally infested with A. sculptum in triplicate. Notably, dry ice (CO2) remained the best atluring ticks in the field (P < 0.001). Benzoic acid should be further investigated since attractant activity was strongly confirmed in both laboratory and field tests. On the other hand, isobutyric acid and R-limonene could be better exploited due to their repellent role revealed by the lab assay, which makes themworthwhile molecules as natural repellents for the management of this tick. aInsect attractants aInsect repellents aOlfactometers aSemiochemicals aTick control aAtrativo aCarrapato aRepelente aAlternative tick control aAmblyomma cajennense sensu lato aField trial aY-tube olfactometer1 aOLIVEIRA FILHO, J. G. de1 aSILVA, F. de O.1 aFERRAZ, A. L. L.1 aMASCARIN, G. M. tVeterinary Parasitologygv. 278, feb. 2020. Article 109036.