02137naa a2200289 a 450000100080000000500110000800800410001902400510006010000210011124501340013226000090026652012380027565300330151365300240154665300310157065300260160165300200162770000150164770000200166270000200168270000200170270000130172270000200173570000230175570000200177877300490179821213682021-01-08       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s00572-020-00948-2DOI1 aNOVAIS, C. B. de  aMycorrhizal networks facilitate the colonization of legume roots by a symbiotic nitrogen-fixing bacterium.h[electronic resource]  c2020  aHere, we assessed whether nitrogen-fixing rhizobia can be transferred to the host legume Glycinemaxby ERM produced by Glomus formosa numisolate CNPAB020 colonizing the grass Urochloa decumbens. An H-bridge experimental system was developed to evaluate the migration of ERM and of the GFP-tagged Bradyrhizobium diazo efficiens USDA 110 strain across an air gap compartment. Mycorrhizal colonization, nodule formation in legumes, and occurrence of the GFP-tagged strain in root nodules were assessed by optical and confocal laser scanning microscopy. In the presence of non-mycorrhizalU. decumbens, legume roots were neither AMF-colonized nor nodulated. In contrast,G. formosa num ERM crossingthe discontinuous compartment connected mycorrhiza U. decumbens and G. maxroots, which showed 30?42% mycorrhizal colonization and 7?11 nodules per plant. Fluorescent B. diazo efficiens cells were detected in 94% ofG. maxroot nodules. Our findings reveal that, besides its main activity in nutrient transfer, ERM produced by AMF may facilitate bacterial translocation and the simultaneous associations of plants with beneficial fungi and bacteria, representing an important structure, functional tothe establishment of symbiotic relationships.  aArbuscular mycorrhizal fungi  aBacterial migration  aCommon mycorrhizal network  aExtraradical mycelium  aPlant symbioses1 aSBRANA, C.1 aJESUS, E. da C.1 aROUWS, L. F. M.1 aGIOVANNETTI, M.1 aAVIO, L.1 aSIQUEIRA, J. O.1 aSILVA, E. M. R. da1 aFARIA, S. M. de  tMycorrhiza, published Online: 25 march 2020.