02035naa a2200265 a 450000100080000000500110000800800410001910000190006024501550007926000090023452012080024365000360145165000190148765000230150665000120152970000180154170000180155970000260157770000230160370000200162670000190164670000150166570000190168077300700169921186842020-01-14       2019    bl uuuu     u00u1 u    #d1 aBARBOSA, A. M.  aSeparation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.h[electronic resource]  c2019  aHancornia speciosahas bioactive molecules such as phenolic compounds which exhibit antioxidant and anti-microbial properties. Nevertheless, the separation of these substances from a medicinal plant can be differentaccording to the extraction process and solvent used. Thus, the present study aimed was to separate phenoliccompounds fromHancornia speciosaleaves in three steps by using a sequential pressurized liquid extraction andevaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to60° C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 ± 0.2%)showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 °C exhibited thehighest amount of total phenolic compounds (347.0 ± 4.12 mg/g extract). Fractionated ethyl acetate extract(FAE) at 60 °C presented improved quantity of totalflavonoids (230.4 ± 3.5 mg/g extract) and rutin content(16.6 ± 0.3 mg/mL extract). The antioxidant activity results for IC50of DPPH indicated that FEE(3.8 ± 0.1 ?g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of mul-tidrug-resistantE. coli.  aTropical and subtropical fruits  aFruta Tropical  aHancornia Speciosa  aMangaba1 aSANTOS, K. S.1 aBORGES, G. R.1 aMUNIZ, A. V. C. da S.1 aMENDONCA, F. M. R.1 aPINHEIRO, M. S.1 aFRANCESCHI, E.1 aDARIVA, C.1 aPADILHA, F. F.  tSeparation and Purification Technologygn. 222, p. 390-395, 2019.