02043nam a2200325 a 450000100080000000500110000800800410001902200220006010000180008224501280010026001380022830000150036650000280038152010310040965000200144065000100146065300250147065300080149565300270150365300100153070000200154070000180156070000220157870000200160070000250162070000220164570000230166770000140169070000130170421167402020-01-07       2019    bl uuuu     u00u1 u    #d  a978-85-0000-000-01 aGARCIA, A. R.  aOsmolaridade ideal para execução do teste hiposmótico no sêmen in natura de ovinos (Ovis aries).h[electronic resource]  aIn: REUNIÃO ANUAL DA ASSOCIAÇÃO BRASILEIRA DE ANDROLOGIA ANIMAL (ABRAA), 4., 2019, Goiânia. Anais... Goiânia: Editora UFMSc2019  ap.160-163.  a7 e 8 de junho de 2019.  aThe study aimed to establish the most suitable osmolarity range of the hypoosmotic solution for testing the membrane integrity in raw semen of rams. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/L) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/L (S10). Nine mature Santa Ines rams (21.5+0.5 months; 72.9+1.6 kg) had semen collected one month apart. After the hypoosmotic swelling test (HOST) the ejaculates were evaluated using a phase-contrast microscope. A total of 200 spermatozoa were counted and sperm cells were classified as spermatozoa with coiled tails (CD, %), strongly coiled tail (CFD, %) or non-coiled tails. According to CD, S1, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P&lt0,05). According to CED, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P&lt0,05). Results suggest that the 75 up to 200 mOsm/L solution would be bestfor using in HOST when raw semen of rams is analyzed.  aEspermatozóide  aOvino  aHypoosmotic solution  aRam  aSolução hiposmótica  aSperm1 aMOURA, A. B. B.1 aROMANELLO, N.1 aFONSECA, J. F. da1 aBRANDÃO, F. Z.1 aPANTOJA, M. H. de A.1 aBARRETO, A. do N.1 aSOUSA, M. A. de P.1 aBOTTA, D.1 aGIRO, A.