02687naa a2200325 a 450000100080000000500110000800800410001902200140006002400650007410000170013924501060015626000090026252017360027165000160200765000110202365300150203465300280204965300230207765300150210065300150211565300290213065300180215965300190217770000190219670000170221570000280223270000190226070000200227977300620229921162552019-12-11       2019    bl uuuu     u00u1 u    #d  a1678-51507 aPesquisa Agropecuária Brasileira, v. 54, e00696, 2019.2DOI1 aROCHA, T. G.  aSerum proteinogram and biochemistry of Holstein cows in the peripartum period.h[electronic resource]  c2019  aIn face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents? concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey?s test. Results were considered significant when P&lt0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.  aBioquímica  aBovino  aMorfologia  aPeríodo de transição  aPeríodo periparto  aPlurípara  aPrimípara  aProteínas de fase aguda  aProteinograma  aVaca Holandesa1 aBORTOLETTO, C.1 aSILVA, D. G.1 aSIMPLÍCIO, K. M. M. G.1 aZAFALON, L. F.1 aFAGLIARI, J. J.  tPesquisa Agropecuária Brasileiragv.39, n.5, maio, 2019.