02229naa a2200277 a 450000100080000000500110000800800410001902400410006010000200010124501360012126000090025752013650026665000230163165000190165465000200167365000270169365000190172065000240173965000220176365000170178565300160180270000200181870000270183870000230186577300630188821152622019-11-29       2019    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1002/ps.54582DOI1 aSILVA, J. do N.  aNovel combination of a biosurfactant with entomopathogenic fungi enhances efficacy against Bemisia whitefly.h[electronic resource]  c2019  aAbstract: Microbial surfactants are multifunctional surface‐active molecules that have been overlooked in formulating microbial biopesticides. We report a novel approach using the biosurfactant rhamnolipid (RML) against the destructive cosmopolitan insect pest Bemisia tabaci, as well as the combined action of RML with aerial conidia of two entomopathogenic fungi, Cordyceps javanica and Beauveria bassiana. RML was also tested as a suspension agent to improve the recovery rate of conidia from solid substrate for fungal preparations. The recovery rate of conidia increased dramatically (two to five times) with RML compared with a standard surfactant (Tween 80). Spraying solutions of 0.075% and 0.1% (w/v) RML on B. tabaci third instar nymphs induced 100% mortality within 4 days. Conidial suspensions at 5 × 106 conidia/mL amended with RML at 0.01% or 0.05% markedly increased nymphal mortalities and considerably reduced LC50. Conidial suspensions of B. bassiana with 0.05% RML added were more effective against whitefly nymphs (87.3% mortality) than C. javanica + RML (51.4% mortality). Our results show that this bacterium‐based RML improved the recovery rate of hydrophobic conidia, and that mixtures of RML with fungal spore suspensions increased their insecticidal activity. © 2019 Society of Chemical Industry.  aBiological control  aBiosurfactants  aCordycipitaceae  aEntomopathogenic fungi  aBemisia Tabaci  aControle Biológico  aFungo Entomógeno  aMosca Branca  aRhamnolipid1 aMASCARIN, G. M.1 aCASTRO, R. de P. V. de1 aCASTILHO, L dos R.  tPest Management Sciencegv. 75, n. 11, p. 2882-2891, 2019.