02094naa a2200325 a 450000100080000000500110000800800410001902200140006002400550007410000210012924501210015026000090027152011200028065000190140065000180141965000160143765000170145365000140147065300290148470000170151370000240153070000260155470000220158070000200160270000190162270000180164170000240165970000210168377300640170421148272019-11-21       2019    bl uuuu     u00u1 u    #d  a1478-64197 ahttps://doi.org/10.1080/14786419.2018.14346392DOI1 aBAUERMEISTER, A.  aPradimicin-IRD from Amycolatopsis sp. IRD-009 and its antimicrobial and cytotoxic activities.h[electronic resource]  c2019  aA new polycyclic antibiotic, pradimicin-IRD, was isolated from actinobacteria Amycolatopsis sp. IRD-009 recovered from soil of Brazilian rainforest undergoing restoration area. This molecule is the major compound produced in solid culture media. The new compound was detected by a focused method of precursor ion (high-performance liquid chromatography coupled to tandem mass spectrometer) developed previously to identify unusual aminoglycosyl sugar moieties. The  compound was isolated and its structure was, therefore, elucidated by high-resolution mass spectrometry, and 1D and 2D nuclear magnetic resonance experiments. Pradimicin- IRD displayed potential antimicrobial activity against Streptococcus agalactiae (MIC  3.1  μg/mL), Pseudomonas aeruginosa (MIC  3.1  μg/ mL) and Staphylococcus aureus (MIC 3.1 μg/mL), and also cytotoxicity against tumour and non-tumour cell lines with IC 50 values ranging from 0.8 μM in HCT-116 colon carcinoma cells to 2.7 μM in MM 200 melanoma cells. Particularly, these biological properties are described for the first time for this chemical class.  aActinobacteria  aAmycolatopsis  aAntibiotics  aAntibiótico  aBactéria  aAntimicrobial properties1 aCALIL, F. A.1 aPINTO, F. das C. L.1 aMEDEIROS, T. C. de T.1 aALMEIDA, L. C. de1 aSILVA, L. J. da1 aMELO, I. S. de1 aZUCCHI, T. D.1 aCOSTA-LOTUFO, L. V.1 aMORAES, L. A. B.  tNatural Product Researchgv. 33, n. 12, p. 1713-1720, 2019.